Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/126087
Title: Optical modulation of neurotransmission using calcium photocurrents through the ion channel LiGluR
Author: Izquierdo Serra, Mercè
Trauner, Dirk
Llobet Berenguer, Artur
Gorostiza Langa, Pablo Ignacio
Keywords: Calci
Neurotransmissió
Òptica
Sinapsi
Calcium
Neural transmission
Optics
Synapses
Issue Date: 21-Mar-2013
Publisher: Frontiers Media
Abstract: A wide range of light-activated molecules (photoswitches and phototriggers) have been used to the study of computational properties of an isolated neuron by acting pre and postsynaptically. However, new tools are being pursued to elicit a presynaptic calcium influx that triggers the release of neurotransmitters, most of them based in calcium-permeable Channelrhodopsin-2 mutants. Here we describe a method to control exocytosis of synaptic vesicles through the use of a light-gated glutamate receptor (LiGluR), which has recently been demonstrated that supports secretion by means of calcium influx in chromaffin cells. Expression of LiGluR in hippocampal neurons enables reversible control of neurotransmission with light, and allows modulating the firing rate of the postsynaptic neuron with the wavelength of illumination. This method may be useful for the determination of the complex transfer function of individual synapses.
Note: Reproducció del document publicat a: https://doi.org/10.3389/fnmol.2013.00003
It is part of: Frontiers In Molecular Neuroscience, 2013, vol. 6, num. 3
URI: http://hdl.handle.net/2445/126087
Related resource: https://doi.org/10.3389/fnmol.2013.00003
ISSN: 1662-5099
Appears in Collections:Articles publicats en revistes (Patologia i Terapèutica Experimental)
Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC))
Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))

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