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http://hdl.handle.net/2445/126223
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DC Field | Value | Language |
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dc.contributor.author | Sanmartí Espinal, Marta | - |
dc.contributor.author | Iavicoli, Patrizia | - |
dc.contributor.author | Calò, Annalisa | - |
dc.contributor.author | Taulés i Marín, Marta | - |
dc.contributor.author | Galve Bosch, Roger | - |
dc.contributor.author | Marco Colás, Ma. Pilar | - |
dc.contributor.author | Samitier i Martí, Josep | - |
dc.date.accessioned | 2018-11-19T18:10:56Z | - |
dc.date.available | 2018-11-19T18:10:56Z | - |
dc.date.issued | 2017-12-13 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | http://hdl.handle.net/2445/126223 | - |
dc.description.abstract | This study aims to improve our understanding of the interaction between olfactory receptors and odorants to develop highly selective biosensing devices. Natural nanovesicles (NVs) from Saccharomyces cerevisiae, ~100 nm in diameter, carrying either the human OR17-40 or the chimpanzee OR7D4 olfactory receptor (OR) tagged with the c-myc epitope at their N-terminus, are presented as model systems to quantify the interaction between odorant and olfactory receptors. The level of expression of olfactory receptors was determined at individual NVs using a novel competitive ELISA immunoassay comparing the values obtained against those from techniques involving the solubilization of cell membrane proteins and the identification of c-myc-carrying receptors. Surface Plasmon Resonance (SPR) measurements on L1 Biacore chips indicate that cognate odorants bind to their Ors, thereby quantifying the approximate number of odorants that interact with a given olfactory receptor. The selectivity of OR17-40-carrying NVs towards helional and OR7D4-carrying NVs towards androstenone has been proven in cross-check experiments with non-specific odorant molecules (heptanal and pentadecalactone, respectively) and in control receptors. | - |
dc.format.extent | 11 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Nature Publishing Group | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.1038/s41598-017-16997-9 | - |
dc.relation.ispartof | Scientific Reports, 2017, vol. 7, p. 17483 | - |
dc.relation.uri | https://doi.org/10.1038/s41598-017-16997-9 | - |
dc.rights | cc-by (c) Sanmartí Espinal, Marta et al., 2017 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es | - |
dc.source | Articles publicats en revistes (Enginyeria Electrònica i Biomèdica) | - |
dc.subject.classification | Olfacte | - |
dc.subject.classification | Biosensors | - |
dc.subject.classification | Olors | - |
dc.subject.classification | Bioenginyeria | - |
dc.subject.other | Smell | - |
dc.subject.other | Biosensors | - |
dc.subject.other | Odors | - |
dc.subject.other | Bioengineering | - |
dc.title | Quantification of interacting cognate odorants with olfactory receptors in nanovesicles | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 677289 | - |
dc.date.updated | 2018-11-19T18:10:56Z | - |
dc.relation.projectID | info:eu-repo/grantAgreement/EC/FP7/228685/EU//BOND | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 29235485 | - |
Appears in Collections: | Articles publicats en revistes (Enginyeria Electrònica i Biomèdica) |
Files in This Item:
File | Description | Size | Format | |
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677289.pdf | 1.58 MB | Adobe PDF | View/Open |
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