Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/128079
Title: Tropomyosin controls sarcomere-like contractions for rigidity sensing and suppressing growth on soft matrices
Author: Wolfenson, Haguy
Meacci, Giovanni
Liu, Shuaimin
Stachowiak, Matthew R.
Iskratsch, Thomas
Ghassemi, Saba
Roca-Cusachs Soulere, Pere
Shaughnessy, Ben O'
Hone, James
Sheetz, Michael P.
Keywords: Matriu extracel·lular
Extracellular matrix
Issue Date: 2016
Publisher: Nature Publishing Group
Abstract: Cells test the rigidity of the extracellular matrix by applying forces to it through integrin adhesions. Recent measurements show that these forces are applied by local micrometre-scale contractions, but how contraction force is regulated by rigidity is unknown. Here we performed high temporal- and spatial-resolution tracking of contractile forces by plating cells on sub-micrometre elastomeric pillars. We found that actomyosin-based sarcomere-like contractile units (CUs) simultaneously moved opposing pillars in net steps of ∼2.5 nm, independent of rigidity. What correlated with rigidity was the number of steps taken to reach a force level that activated recruitment of α-actinin to the CUs. When we removed actomyosin restriction by depleting tropomyosin 2.1, we observed larger steps and higher forces that resulted in aberrant rigidity sensing and growth of non-transformed cells on soft matrices. Thus, we conclude that tropomyosin 2.1 acts as a suppressor of growth on soft matrices by supporting proper rigidity sensing.
Note: Versió postprint del document publicat a: https://doi.org/10.1038/ncb3277
It is part of: Nature Cell Biology, 2016, vol. 18, p. 33-42
Related resource: https://doi.org/10.1038/ncb3277
URI: http://hdl.handle.net/2445/128079
ISSN: 1465-7392
Appears in Collections:Articles publicats en revistes (Biomedicina)

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