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|Title:||Tropomyosin controls sarcomere-like contractions for rigidity sensing and suppressing growth on soft matrices|
Stachowiak, Matthew R.
Roca-Cusachs Soulere, Pere
Shaughnessy, Ben O'
Sheetz, Michael P.
|Publisher:||Nature Publishing Group|
|Abstract:||Cells test the rigidity of the extracellular matrix by applying forces to it through integrin adhesions. Recent measurements show that these forces are applied by local micrometre-scale contractions, but how contraction force is regulated by rigidity is unknown. Here we performed high temporal- and spatial-resolution tracking of contractile forces by plating cells on sub-micrometre elastomeric pillars. We found that actomyosin-based sarcomere-like contractile units (CUs) simultaneously moved opposing pillars in net steps of ∼2.5 nm, independent of rigidity. What correlated with rigidity was the number of steps taken to reach a force level that activated recruitment of α-actinin to the CUs. When we removed actomyosin restriction by depleting tropomyosin 2.1, we observed larger steps and higher forces that resulted in aberrant rigidity sensing and growth of non-transformed cells on soft matrices. Thus, we conclude that tropomyosin 2.1 acts as a suppressor of growth on soft matrices by supporting proper rigidity sensing.|
|Note:||Versió postprint del document publicat a: https://doi.org/10.1038/ncb3277|
|It is part of:||Nature Cell Biology, 2016, vol. 18, p. 33-42|
|Appears in Collections:||Articles publicats en revistes (Biomedicina)|
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