Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/128079
Title: | Tropomyosin controls sarcomere-like contractions for rigidity sensing and suppressing growth on soft matrices |
Author: | Wolfenson, Haguy Meacci, Giovanni Liu, Shuaimin Stachowiak, Matthew R. Iskratsch, Thomas Ghassemi, Saba Roca-Cusachs Soulere, Pere Shaughnessy, Ben O' Hone, James Sheetz, Michael P. |
Keywords: | Matriu extracel·lular Extracellular matrix |
Issue Date: | 2016 |
Publisher: | Nature Publishing Group |
Abstract: | Cells test the rigidity of the extracellular matrix by applying forces to it through integrin adhesions. Recent measurements show that these forces are applied by local micrometre-scale contractions, but how contraction force is regulated by rigidity is unknown. Here we performed high temporal- and spatial-resolution tracking of contractile forces by plating cells on sub-micrometre elastomeric pillars. We found that actomyosin-based sarcomere-like contractile units (CUs) simultaneously moved opposing pillars in net steps of ∼2.5 nm, independent of rigidity. What correlated with rigidity was the number of steps taken to reach a force level that activated recruitment of α-actinin to the CUs. When we removed actomyosin restriction by depleting tropomyosin 2.1, we observed larger steps and higher forces that resulted in aberrant rigidity sensing and growth of non-transformed cells on soft matrices. Thus, we conclude that tropomyosin 2.1 acts as a suppressor of growth on soft matrices by supporting proper rigidity sensing. |
Note: | Versió postprint del document publicat a: https://doi.org/10.1038/ncb3277 |
It is part of: | Nature Cell Biology, 2016, vol. 18, p. 33-42 |
URI: | http://hdl.handle.net/2445/128079 |
Related resource: | https://doi.org/10.1038/ncb3277 |
ISSN: | 1465-7392 |
Appears in Collections: | Articles publicats en revistes (Biomedicina) Publicacions de projectes de recerca finançats per la UE |
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