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Title: | Trichomonicidal and parasite membrane damaging activity of bidesmosic saponins from Manilkara rufula |
Author: | Brum Vieira, Patricia de Feijo Silva, Nicolas Luiz Braz Menezes, Camila Vanusa da Silva, Marcia Brentan Silva, Denise Peporine Lopes, Norberto Macedo, Alexandre José Bastida Armengol, Jaume Tasca, Tiana |
Keywords: | Resistència als medicaments Efecte dels medicaments sobre els microorganismes Malalties de transmissió sexual Membranes cel·lulars Drug resistance Effect of drugs on microorganisms Sexually transmitted diseases Cell membranes |
Issue Date: | 30-Nov-2017 |
Publisher: | Public Library of Science (PLoS) |
Abstract: | The infection caused by Trichomonas vaginalis is the most common but overlooked nonviral sexually transmitted disease worldwide. Treatment relies on one class of drugs, the 5nitroimidazoles, but resistance is widespread. New drugs are urgently needed. We reported the effect of crude and purified saponin fractions of Manilkara rufula against Trichomonas vaginalis. The compound responsible for antitrichomonal activity was isolated and identified as an uncommon bidesmosic saponin, Mi-saponin C. This saponin eliminated parasite viability without toxicity against the human vaginal epithelial line (HMVII). In addition, the isolated saponin fraction improved the metronidazole effect against a metronidazole-resistant isolate and dramatically reduced the cytoadherence of T. vaginalis to human cells. Investigation of the mechanism of death showed that the saponin fraction induced the parasite death due to profound membrane damage, inducing a disturbance of intracellular content without nuclear damage. To the best of our knowledge, this is the first report of antitrichomonal activity in the bidesmosic saponins of Manilkara rufula. |
Note: | Reproducció del document publicat a: https://doi.org/10.1371/journal.pone.0188531 |
It is part of: | PLoS One, 2017, vol. 12, num. 11, p. e0188531 |
URI: | http://hdl.handle.net/2445/130925 |
Related resource: | https://doi.org/10.1371/journal.pone.0188531 |
ISSN: | 1932-6203 |
Appears in Collections: | Articles publicats en revistes (Biologia, Sanitat i Medi Ambient) |
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