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Title: Large-area biomolecule nanopatterns on diblock copolymer surfaces for cell adhesion studies
Author: Hortigüela, Verónica
Larrañaga, Enara
Lagunas, Anna
Acosta, Gerardo A.
Albericio, Fernando
Andilla, Jordi
Loza-Alvarez, Pablo
Martínez, Elena
Keywords: Receptors cel·lulars
Cell receptors
Issue Date: 9-Apr-2019
Publisher: MDPI
Citation: ISSN
Abstract: Cell membrane receptors bind to extracellular ligands, triggering intracellular signal transduction pathways that result in specific cell function. Some receptors require to be associated forming clusters for effective signaling. Increasing evidences suggest that receptor clustering is subjected to spatially controlled ligand distribution at the nanoscale. Herein we present a method to produce in an easy, straightforward process, nanopatterns of biomolecular ligands to study ligand–receptor processes involving multivalent interactions. We based our platform in self-assembled diblock copolymers composed of poly(styrene) (PS) and poly(methyl methacrylate) (PMMA) that form PMMA nanodomains in a closed-packed hexagonal arrangement. Upon PMMA selective functionalization, biomolecular nanopatterns over large areas are produced. Nanopattern size and spacing can be controlled by the composition of the block-copolymer selected. Nanopatterns of cell adhesive peptides of different size and spacing were produced, and their impact in integrin receptor clustering and the formation of cell focal adhesions was studied. Cells on ligand nanopatterns showed an increased number of focal contacts, which were, in turn, more matured than those found in cells cultured on randomly presenting ligands. These findings suggest that our methodology is a suitable, versatile tool to study and control receptor clustering signaling and downstream cell behavior through a surface-based ligand patterning technique.
It is part of: Nanomaterials, 2019, vol. 9, p. 579
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Appears in Collections:Articles publicats en revistes (Electrònica)
Publicacions de projectes de recerca finançats per la UE
Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC))
Articles publicats en revistes (Química Inorgànica i Orgànica)

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