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Title: Probing cellular mechanics with acoustic force spectroscopy
Author: Sorkin, Raya
Bergamaschi, Giulia
Kansma, Douwe
Brand, Guy
Dekel, Elya
Ofir Birin, Yifat
Rudik, Ariel
Gironella, Marta
Ritort Farran, Fèlix
Regev Rudzki, Neta
Roos, Wouter H.
Wuite, Gijs J. L.
Keywords: Acústica
Fisiologia cel·lular
Cell physiology
Issue Date: Aug-2018
Publisher: American Society for Cell Biology
Abstract: A large number of studies demonstrate that cell mechanics and pathology are intimately linked. In particular, deformability of red blood cells (RBCs) is key to their function and is dramatically altered in the time course of diseases such as anemia and malaria. Due to the physiological importance of cell mechanics, many methods for cell mechanical probing have been developed. While single-cell methods provide very valuable information, they are often technically challenging and lack the high data throughput needed to distinguish differences in heterogeneous populations, while fluid-flow high-throughput methods miss the accuracy to detect subtle differences. Here we present a new method for multiplexed singlecell mechanical probing using acoustic force spectroscopy (AFS). We demonstrate that mechanical differences induced by chemical treatments of known effect can be measured and quantified. Furthermore, we explore the effect of extracellular vesicles (EVs) uptake on RBC mechanics and demonstrate that EVs uptake increases RBC deformability. Our findings demonstrate the ability of AFS to manipulate cells with high stability and precision and pave the way to further new insights into cellular mechanics and mechanobiology in health and disease, as well as potential biomedical applications.
Note: Reproducció del document publicat a:
It is part of: Molecular Biology of the Cell, 2018, vol. 29, p. 2005-2011
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ISSN: 1059-1524
Appears in Collections:Articles publicats en revistes (Física de la Matèria Condensada)

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