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Title: Analysis of corticosteroids in samples of animal origin using QuEChERS and ultrahigh-performance liquid chromatography coupled to high-resolution mass spectrometry.
Author: Beguiristain Seguí, Iñaki
Alongi, Angelina
Rúbies Prat, Antoni
Granados i Juan, Mercè
Keywords: Cromatografia de líquids
Espectrometria de masses
Liquid chromatography
Mass spectrometry
Issue Date: 26-Nov-2018
Publisher: Springer Verlag
Abstract: A rapid and sensitive method for the confirmatory analysis of 8 synthetic corticosteroids (betamethasone, dexamethasone, prednisolone, 6 methyl prednisolone, triamcinolone, flumethasone, beclomethasone, fluocinolone acetonide) is proposed. The method is useful for detecting illegal treatments in different animal species. It consists of an extraction and clean-up using the Quick Easy Cheap Effective Rugged Safe (QuEChERS) strategy. Quantitative determination is achieved by ultra-high performance liquid chromatography coupled to high-resolution mass spectrometry with heated electrospray ionization in negative mode. Quantification is performed using surrogate matrix matched standard calibration curve with dexamethasone-D4 as the internal standard. The method was validated for analyzing liver samples according to the criteria established by Decision 2002/657/EC. Linearity was assessed in the 1-10 μg kg-1 range and linear correlation coefficients were over 0.99 for all the analytes. CCα ranged from 0.04 to 0.16 μg kg-1 for substances without maximum residue limit. The method allows confident quantification and confirmation of corticosteroids in liver samples, and its simplicity makes it suitable for analyzing large numbers of samples.
Note: Versió postprint del document publicat a:
It is part of: Analytical and Bioanalytical Chemistry, 2018, vol. 411, num. 2, p. 449-457
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ISSN: 1618-2642
Appears in Collections:Articles publicats en revistes (Enginyeria Química i Química Analítica)

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