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http://hdl.handle.net/2445/135119
Title: | Analysis of corticosteroids in samples of animal origin using QuEChERS and ultrahigh-performance liquid chromatography coupled to high-resolution mass spectrometry. |
Author: | Beguiristain Seguí, Iñaki Alongi, Angelina Rúbies Prat, Antoni Granados i Juan, Mercè |
Keywords: | Cromatografia de líquids Espectrometria de masses Liquid chromatography Mass spectrometry |
Issue Date: | 26-Nov-2018 |
Publisher: | Springer Verlag |
Abstract: | A rapid and sensitive method for the confirmatory analysis of 8 synthetic corticosteroids (betamethasone, dexamethasone, prednisolone, 6 methyl prednisolone, triamcinolone, flumethasone, beclomethasone, fluocinolone acetonide) is proposed. The method is useful for detecting illegal treatments in different animal species. It consists of an extraction and clean-up using the Quick Easy Cheap Effective Rugged Safe (QuEChERS) strategy. Quantitative determination is achieved by ultra-high performance liquid chromatography coupled to high-resolution mass spectrometry with heated electrospray ionization in negative mode. Quantification is performed using surrogate matrix matched standard calibration curve with dexamethasone-D4 as the internal standard. The method was validated for analyzing liver samples according to the criteria established by Decision 2002/657/EC. Linearity was assessed in the 1-10 μg kg-1 range and linear correlation coefficients were over 0.99 for all the analytes. CCα ranged from 0.04 to 0.16 μg kg-1 for substances without maximum residue limit. The method allows confident quantification and confirmation of corticosteroids in liver samples, and its simplicity makes it suitable for analyzing large numbers of samples. |
Note: | Versió postprint del document publicat a: https://doi.org/10.1007/s00216-018-1459-y |
It is part of: | Analytical and Bioanalytical Chemistry, 2018, vol. 411, num. 2, p. 449-457 |
URI: | http://hdl.handle.net/2445/135119 |
Related resource: | https://doi.org/10.1007/s00216-018-1459-y |
ISSN: | 1618-2642 |
Appears in Collections: | Articles publicats en revistes (Enginyeria Química i Química Analítica) |
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686311.pdf | 398.55 kB | Adobe PDF | View/Open |
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