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DC Field | Value | Language |
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dc.contributor.author | López Cristoffanini, Camilo Alonso | - |
dc.contributor.author | Serrat Gurrera, Xavier | - |
dc.contributor.author | Jáuregui Pallarés, Olga | - |
dc.contributor.author | Nogués Mestres, Salvador | - |
dc.contributor.author | López Carbonell, Marta | - |
dc.date.accessioned | 2019-09-18T13:59:20Z | - |
dc.date.available | 2019-09-18T13:59:20Z | - |
dc.date.issued | 2019-06-07 | - |
dc.identifier.issn | 1664-462X | - |
dc.identifier.uri | http://hdl.handle.net/2445/140443 | - |
dc.description.abstract | Gibberellins (GAs) are a very important group of phytohormones involved in seed germination, vegetative growth, flowering, and fruit development, being only 4 of the 136 known bioactives: GA1, GA3, GA4 and GA7. It has been evidenced that mutations in the OsGA20ox-2 gene produce rice (Oryza sativa) dwarf varieties, which were one of the main pillars of the green revolution. In this work two main objectives were proposed: (i) develop a rapid and broad phytohormone profiling method and (ii) to study the effects on the GA content of the GA20ox-2 mutation in several rice developmental stages using three varieties (tall variety, elite variety, mutated variety). A phytohormone extraction using an SPE step and HPLC-MS/MS detection using a QqQ instrument was determined which resulted in limits of detection (LOD) and limits of quantification (LOQ) for GAs that varied between 0.1-0.7 and 0.3-2.3 pg/g of rice sample respectively, allowing highly sensitive phytohormones detection in samples. Moreover, a good reproducibility was obtained for the GAs as relative standard deviations (RSD) for a 40 ng/mL pattern varied between 0.3-0.9%. Notoriously, GA1 was absent in the coleoptile and GA4 was the GA with higher content in the majority of developmental stages. We also observed a huge large content increment increase of the 4 bioactive GAs in the internode of the flag leaf of the mutated variety that allowinged it to attain reach the same height as the elite variety. Therefore, we provide a rapid and broad phytohormonal profiling method and evidence that the GA20ox-2 mutation is not the only factor generating dwarf varieties. To our knowledge, this is the first study that it has been reported such a high number of analyzed gibberellins at the same time in rice (Oryza sativa L. ssp. japonica)Oryza sativa japonica). | - |
dc.format.extent | 14 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Frontiers Media | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.3389/fpls.2019.00733 | - |
dc.relation.ispartof | Frontiers in Plant Science, 2019, vol. 10, p. 733-000 | - |
dc.relation.uri | https://doi.org/10.3389/fpls.2019.00733 | - |
dc.rights | cc-by (c) López Cristoffanini, Camilo Alonso et al., 2019 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es | - |
dc.source | Articles publicats en revistes (Biologia Evolutiva, Ecologia i Ciències Ambientals) | - |
dc.subject.classification | Arròs | - |
dc.subject.classification | Biotecnologia alimentària | - |
dc.subject.other | Rice | - |
dc.subject.other | Food biotechnology | - |
dc.title | Phytohormone Profiling method for rice: effects of GA20ox mutation on the gibberellin content of japonica rice varieties | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 683732 | - |
dc.date.updated | 2019-09-18T13:59:20Z | - |
dc.relation.projectID | info:eu-repo/grantAgreement/EC/H2020/678168/EU//NEURICE | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 31231411 | - |
Appears in Collections: | Articles publicats en revistes (Biologia Evolutiva, Ecologia i Ciències Ambientals) |
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File | Description | Size | Format | |
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683732.pdf | 8.11 MB | Adobe PDF | View/Open |
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