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Title: | Molecular interactions between warfarin and human (HSA) or bovine(BSA) serum albumin evaluated by isothermal titration calorimetry(ITC), fluorescence spectrometry (FS) and frontal analysis capillaryelectrophoresis (FA/CE) |
Author: | Ràfols Llach, Clara Amézqueta, Susana Fuguet i Jordà, Elisabet Bosch, Elisabeth |
Keywords: | Anticoagulants (Medicina) Electroforesi capil·lar Anticoagulants (Medicine) Capillary electrophoresis |
Issue Date: | 18-Dec-2017 |
Publisher: | Elsevier B.V. |
Abstract: | Interaction thermodynamics between warfarin, a very popular anticoagulant, and Sudlow I binding siteof human (HSA) or bovine (BSA) serum albumin have been examined in strictly controlled experimentalconditions (HEPES buffer 50 mM, pH 7.4 and 25◦C) by means of isothermal titration calorimetry (ITC),fluorescence spectrometry (FS) and frontal analysis capillary electrophoresis (FA/CE). Each technique isbased on measurements of a different property of the biochemical system, and then the results allow acritical discussion about the suitability of each approach to estimate the drug-protein binding parameters.The strongest interaction step is properly evaluated by the three assayed approaches being the derivedbinding constants strongly consistent: from 4 × 104to 7 × 104for HSA and from 0.8 × 105to 1.2 × 105forBSA. Binding enthalpy variations also show consistent results: −5.4 and −5.6 Kcal/mol for HSA and −4.3and −3.7 Kcal/mol for BSA, as measured by ITC and FS, respectively. Further high order interaction eventsfor both albumins are detected only by FA/CE. |
Note: | Versió postprint del document publicat a: https://doi.org/10.1016/j.jpba.2017.12.008 |
It is part of: | Journal of Pharmaceutical and Biomedical Analysis, 2017, vol. 150, p. 452-459 |
URI: | http://hdl.handle.net/2445/143335 |
Related resource: | https://doi.org/10.1016/j.jpba.2017.12.008 |
ISSN: | 0731-7085 |
Appears in Collections: | Articles publicats en revistes (Enginyeria Química i Química Analítica) |
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