Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/143720
Title: Hypothalamic endocannabinoids inversely correlate with the development of diet-induced obesity in male and female mice
Author: Miralpeix, Cristina
Fosch, Anna
Casas, Josefina
Baena, Jose Miguel
Herrero Rodríguez, Laura
Serra i Cucurull, Dolors
Rodríguez-Rodríguez, Rosalía
Casals i Farré, Núria
Keywords: Hipotàlem
Dimorfisme sexual en els animals
Teixit adipós
Obesitat
Hypothalamus
Sexual dimorphism (Animals)
Adipose tissues
Obesity
Issue Date: 2019
Publisher: American Society for Biochemistry and Molecular Biology
Abstract: The endocannabinoid (eCB) system regulates energy homeostasis and is linked to obesity development. However, the exact dynamic and regulation of eCBs in the hypothalamus during obesity progression remain incompletely described and understood. Our study examined the time course of responses in two hypothalamic eCBs, 2-arachidonoylglycerol (2-AG) and arachidonoylethanolamine (AEA), in male and female mice during diet-induced obesity and explored the association of eCB levels with changes in brown adipose tissue (BAT) thermogenesis and body weight. We fed mice a high-fat diet (HFD), which induced a transient increase (substantial at 7 days) in hypothalamic eCBs, followed by a progressive decrease to basal levels with a long-term HFD. This transient rise at early stages of obesity is considered a physiologic compensatory response to BAT thermogenesis, which is activated by diet surplus. The eCB dynamic was sexually dimorphic: hypothalamic eCBs levels were higher in female mice, who became obese at later time points than males. The hypothalamic eCBs time course positively correlated with thermogenesis activation, but negatively matched body weight, leptinemia, and circulating eCB levels. Increased expression of eCB-synthetizing enzymes accompanied the transient hypothalamic eCB elevation. Icv injection of eCB did not promote BAT thermogenesis; however, administration of thermogenic molecules, such as central leptin or a peripheral β3-adrenoreceptor agonist, induced a significant increase in hypothalamic eCBs, suggesting a directional link from BAT thermogenesis to hypothalamic eCBs. This study contributes to the understanding of hypothalamic regulation of obesity. Keywords: hypothalamus, sexual dimorphism, brown adipose tissue
Note: Reproducció del document publicat a: https://doi.org/10.1194/jlr.M092742
It is part of: Journal of Lipid Research, 2019, vol. 60, p. 1260-1269
URI: http://hdl.handle.net/2445/143720
Related resource: https://doi.org/10.1194/jlr.M092742
ISSN: 0022-2275
Appears in Collections:Articles publicats en revistes (Bioquímica i Fisiologia)

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