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Title: Improved Alere Determine Lipoarabinomannan Antigen Detection Test for the Diagnosis of Human and Bovine Tuberculosis by Manipulating Urine and Milk
Author: García, Juan Ignacio
Kelley, Holden V.
Meléndez, Johanna
Alvarez de León, Rosa Alejandra
Castillo, Alejandra
Sidiki, Sabeen
Yusoof, Kizil A.
Nunes, Elizabete
López Téllez, Cesar
Mejía Villatoro, Carlos Rodolfo
Ikeda, Janet
García-Basteiro, Alberto L.
Wang, Shu-Hua
Torrelles, Jordi B.
Keywords: Tuberculosi
Diagnòstic immunològic
Issue Date: 29-Nov-2019
Publisher: Nature Publishing Group
Abstract: Tuberculosis (TB) disease still kills 1-person every 21-seconds. Few TB diagnostic tests are considered truly appropriate for point of care settings. The WHO-endorsed immunodiagnostic Alere Determine Lipoarabinomannan Ag-test (LAM-test) detects Mycobacterium tuberculosis complex LAM in urine, and its use is recommended for TB diagnosis among HIV co-infected individuals with low CD4 T-cell counts. Here we found that a simple 15-minute enzymatic treatment at room temperature of LAM-spiked urine with \xCE\xB1-mannosidase (for human TB), and LAM-spiked milk with combined lactase and caseinase (for bovine TB), enhanced 10-fold the detection levels of the LAM-test and thus, improved the detection of LAM by the LAM-test in urine and milk that otherwise could be missed in the field. Future separate clinical research studies specifically designed to address the potential of these findings are required.
Note: Reproducció del document publicat a:
It is part of: Scientific Reports, 2019, vol. 9, p. 18012
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ISSN: 2045-2322
Appears in Collections:Articles publicats en revistes (ISGlobal)

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