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|Title:||Improved Alere Determine Lipoarabinomannan Antigen Detection Test for the Diagnosis of Human and Bovine Tuberculosis by Manipulating Urine and Milk|
|Author:||García, Juan Ignacio|
Kelley, Holden V.
Alvarez de León, Rosa Alejandra
Yusoof, Kizil A.
López Téllez, Cesar
Mejía Villatoro, Carlos Rodolfo
García-Basteiro, Alberto L.
Torrelles, Jordi B.
|Publisher:||Nature Publishing Group|
|Abstract:||Tuberculosis (TB) disease still kills 1-person every 21-seconds. Few TB diagnostic tests are considered truly appropriate for point of care settings. The WHO-endorsed immunodiagnostic Alere Determine Lipoarabinomannan Ag-test (LAM-test) detects Mycobacterium tuberculosis complex LAM in urine, and its use is recommended for TB diagnosis among HIV co-infected individuals with low CD4 T-cell counts. Here we found that a simple 15-minute enzymatic treatment at room temperature of LAM-spiked urine with \xCE\xB1-mannosidase (for human TB), and LAM-spiked milk with combined lactase and caseinase (for bovine TB), enhanced 10-fold the detection levels of the LAM-test and thus, improved the detection of LAM by the LAM-test in urine and milk that otherwise could be missed in the field. Future separate clinical research studies specifically designed to address the potential of these findings are required.|
|Note:||Reproducció del document publicat a: http://dx.doi.org/10.1038/s41598-019-54537-9|
|It is part of:||Scientific Reports, 2019, vol. 9, p. 18012|
|Appears in Collections:||Articles publicats en revistes (ISGlobal)|
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