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Title: Non-centrosomal nucleation mediated by augmin organizes microtubules in post-mitotic neurons and controls axonal microtubule polarity
Author: Sánchez Huerta, Carlos
Freixo, Francisco
Viais, Ricardo
Lacasa Salavert, Cristina
Soriano García, Eduardo
Lüders, Jens
Keywords: Neurones
Diferenciació cel·lular
Cell diferentiation
Issue Date: 13-Jul-2016
Publisher: Nature Publishing Group
Abstract: Neurons display a highly polarized microtubule network that mediates trafficking throughout the extensive cytoplasm and is crucial for neuronal differentiation and function. In newborn migrating neurons, the microtubule network is organized by the centrosome. During neuron maturation, however, the centrosome gradually loses this activity, and how microtubules are organized in more mature neurons remains poorly understood. Here, we demonstrate that microtubule organization in post-mitotic neurons strongly depends on non-centrosomal nucleation mediated by augmin and by the nucleator γTuRC. Disruption of either complex not only reduces microtubule density but also microtubule bundling. These microtubule defects impair neurite formation, interfere with axon specification and growth, and disrupt axonal trafficking. In axons augmin does not merely mediate nucleation of microtubules but ensures their uniform plus end-out orientation. Thus, the augmin-γTuRC module, initially identified in mitotic cells, may be commonly used to generate and maintain microtubule configurations with specific polarity.
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It is part of: Nature Communications, 2016, num. 7, p. 12187
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ISSN: 2041-1723
Appears in Collections:Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia)
Articles publicats en revistes (Institut de Recerca Biomèdica (IRB Barcelona))

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