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http://hdl.handle.net/2445/164271
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DC Field | Value | Language |
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dc.contributor.author | Pérez-Burgos, Rafael | - |
dc.contributor.author | Grzelak, Edyta | - |
dc.contributor.author | Gokce, Gultekin | - |
dc.contributor.author | Saurina, Javier | - |
dc.contributor.author | Barbosa Torralbo, José | - |
dc.contributor.author | Barrón Bueno, Dolores | - |
dc.date.accessioned | 2020-06-04T11:21:47Z | - |
dc.date.available | 2020-06-04T11:21:47Z | - |
dc.date.issued | 2012-06-15 | - |
dc.identifier.issn | 1570-0232 | - |
dc.identifier.uri | http://hdl.handle.net/2445/164271 | - |
dc.description.abstract | This work was focused on the comparison of two clean-up methods to be used for the simultaneous determination of seven cephalosporins in cow muscle. In particular, the performance of novel dispersive solid phase extraction (d-SPE) procedures based on QuEChERS methodologies was assessed and compared with conventional SPE. The separation and detection of the analytes using both methods was carried out by LC-MS/MS to reach enough sensitivity to be compatible with the detection of the maximum residue limits (MRL) of cephalosporins as regulated by EU directives. The optimization of the clean-up step relied on experimental design in order to find the most suitable conditions with a reduced number of assays. Besides, multi-objective responses were used to reach an overall compromise in the recovery of all analytes simultaneously. The validation of the two methods was done according to the Directive 2002/657/EC. Linearity, decision limit, detection capability, detection and quantification limits (4-50 μg kg⁻¹), precision (RSD less than 15% except for PIR) and recoveries were determined and adequate results with comparable values using QuEChERS and SPE methodologies. LOQ were better for SPE method (0.1-10 μg kg⁻¹) but both methods show LOQ below MRL values. Precision was slightly better for the QuEChERS method, that also presents better recoveries, higher than 85% except for cephalexin. | - |
dc.format.extent | 9 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier B.V. | - |
dc.relation.isformatof | Versió postprint del document publicat a: https://doi.org/10.1016/j.jchromb.2012.05.002 | - |
dc.relation.ispartof | Journal Of Chromatography B-Analytical Technologies In The Biomedical And Life Sciences, 2012, vol. 899, p. 57-65 | - |
dc.relation.uri | https://doi.org/10.1016/j.jchromb.2012.05.002 | - |
dc.rights | (c) Elsevier B.V., 2012 | - |
dc.source | Articles publicats en revistes (Nutrició, Ciències de l'Alimentació i Gastronomia) | - |
dc.subject.classification | Cefalosporines | - |
dc.subject.classification | Química analítica | - |
dc.subject.classification | Espectrometria de masses | - |
dc.subject.classification | Medicaments antibacterians | - |
dc.subject.other | Cephalosporines | - |
dc.subject.other | Analytical chemistry | - |
dc.subject.other | Mass spectrometry | - |
dc.subject.other | Antibacterial agents | - |
dc.title | QuEChERS methodologies as an alternative to solid phase extraction (SPE) for the determination and characterisation of residues of cephalosporins in beef muscle using LC-MS/MS | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/acceptedVersion | - |
dc.identifier.idgrec | 609017 | - |
dc.date.updated | 2020-06-04T11:21:47Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 22658467 | - |
Appears in Collections: | Articles publicats en revistes (Nutrició, Ciències de l'Alimentació i Gastronomia) |
Files in This Item:
File | Description | Size | Format | |
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609017.pdf | 497.93 kB | Adobe PDF | View/Open |
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