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Title: Marinobacter maroccanus sp. nov., a moderately halophilic bacterium isolated from a saline soil.
Author: Boujida, Nadia
Palau, Montserrat
Charfi, Saoulajan
Manresa Presas, Ma. Ángeles (María Ángeles)
Skali Senhaji, Nadia
Abrini, Jamal
Miñana i Galbis, David
Keywords: Genètica bacteriana
Taxonomia (Biologia)
Microbiologia dels sòls
Bacterial genetics
Taxonomy (Biology)
Soil microbiology
Issue Date: Jan-2019
Publisher: Society for General Microbiology
Abstract: During the taxonomic investigation of exopolymer producing halophilic bacteria, a rod shaped, motile, Gram-stain-negative, aerobic, halophilic bacterium, designated strain N4<sup>T</sup>, was isolated from a natural saline soil located in the northern Morocco. The optimal growth of the isolate was at 30-37 ºC and at pH 6.0-9.0, in the presence of 5-7% (w/v) NaCl. Useful tests for the phenotypic differentiation of strain N4<sup>T</sup> from other <i>Marinobacter</i> species included α-chymotrypsin and α-glucosidase activities and the carbohydrate assimilation profile. The major fatty acids detected in strain N4<sup>T</sup> were C18:1 ω9c, C16:0, and C16:1 ω7c/C15:0 iso 2-OH. Sequence analysis of the 16S rRNA indicated that strain N4<sup>T</sup> belonged to the genus <i>Marinobacter</i> and was closely related to <i>Marinobacter adhaerens</i> NC17506<sup>T</sup> (99.04%), <i>Marinobacter salsuginis</i> SD-14B<sup>T</sup> (98.97%), and <i>Marinobacter flavimaris</i> SW-145<sup>T</sup> (98.36%). Phylogenetic analysis of the rpoD gene sequence also showed that the nearest neighbours of strain N4<sup>T</sup> were <i>M. adhaerens</i> (90.63%) and <i>M. salsuginis</i> (91.13%). Strain N4<sup>T</sup> showed 87.98% similarity in the average nucleotide identity (ANI) with <i>M. flavimaris</i> and <i>M. salsuginis</i>, and 87.47% with <i>M. adhaerens</i>. In the in-silico genome-to-genome distance (GGD), strain N4<sup>T</sup> showed DNA-DNA hybridization (DDH) values of 33.30% with <i>M. adhaerens</i>, 34.60% with <i>M. flavimaris</i> and 34.70% with <i>M. salsuginis</i>. DNA G+C content of N4<sup>T</sup> was 57.3 mol%. Based on the results of phenotypic characterization, phylogenetic analysis and genome comparison, strain N4<sup>T</sup> represents a novel species of the genus <i>Marinobacter</i>, for which the name <i>Marinobacter maroccanus</i> sp. nov. is proposed. The type strain is N4<sup>T</sup> (=CECT 9525<sup>T</sup>=LMG 30466<sup>T</sup>).
Note: Versió postprint del document publicat a:
It is part of: International Journal of Systematic and Evolutionary Microbiology, 2019, vol. 69, num. 1, p. 227-234
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ISSN: 1466-5026
Appears in Collections:Articles publicats en revistes (Biologia, Sanitat i Medi Ambient)

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