Please use this identifier to cite or link to this item:
|Title:||Effects of dietary carbohydrate on hepatic de novo lipogenesis in European seabass (Dicentrarchus labrax L.)|
Carvalho, Rui A.
Metón Teijeiro, Isidoro
Pardal, Miguel A.
Baanante, Isabel V.
Jones, John G.
|Publisher:||American Society for Biochemistry and Molecular Biology|
|Abstract:||Farmed seabass have higher adiposity than their wild counterparts and this is often attributed to carbohydrate (CHO) feeding. Whether this reflects a reduction in fat oxidation, increased de novo lipogenesis (DNL), or both, is not known. To study the effects of high CHO diets on hepatic TG biosynthesis, hepatic TG deuterium (2H) enrichment was determined following 6 days in 2H-enriched tank water for fish fed with a no-CHO control diet (CTRL), and diets with digestible starch (DS) and raw starch (RS). Hepatic fractional synthetic rates (FSRs, percent per day-1) were calculated for hepatic TG-glyceryl and FA moieties through 2H NMR analysis. Glyceryl FSRs exceeded FA FSRs in all cases, indicating active cycling. DS fish did not show increased lipogenic potential compared to CTRL. RS fish had lower glyceryl FSRs compared with the other diets and negligible levels of FA FSRs despite similar hepatic TG levels to CTRL. DS-fed fish showed higher activity for enzymes that can provide NADPH for lipogenesis, relative to CTRL in the case of glucose-6-phosphate dehydrogenase (G6PDH) and relative to RS for both G6PDH and 6-phosphogluconate dehydrogenase. This approach indicated that elevated hepatic adiposity from DS feeding was not attributable to increased DNL.|
|Note:||Reproducció del document publicat a: https://doi.org/10.1194/jlr.M067850|
|It is part of:||Journal of Lipid Research, 2016, vol. 57, num. 7, p. 1264-1272|
|Appears in Collections:||Articles publicats en revistes (Bioquímica i Fisiologia)|
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.