Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/168584
Title: Usefulness of Housekeeping Genes for the Diagnosis of Helicobacter pylori Infection, Strain Discrimination and Detection of Multiple Infection
Author: Palau de Miguel, Montserrat
Kulmann, Marcos
Ramírez-Lázaro, María José
Lario, Sergio
Quilez, María Elisa
Campo, Rafael
Piqué i Clusella, Núria
Calvet, Xavier
Miñana i Galbis, David
Keywords: Helicobacteri pilòric
Genètica bacteriana
Infeccions
Helicobacter pylori
Bacterial genetics
Infections
Issue Date: 2016
Publisher: John Wiley & Sons
Abstract: <b>Background:</b> <i>Helicobacter pylori</i> infects human stomachs of over half the world's population, evades the immune response and establishes a chronic infection. Although most people remains asymptomatic, duodenal and gastric ulcers, MALT lymphoma and progression to gastric cancer could be developed. Several virulence factors such as flagella, lipopolysaccharide, adhesins and especially the vacuolating cytotoxin VacA and the oncoprotein CagA have been described for <i>H. pylori</i>. Despite the extensive published data on <i>H. pylori</i>, more research is needed to determine new virulence markers, the exact mode of transmission or the role of multiple infection. <b>Materials and Methods:</b> Amplification and sequencing of six housekeeping genes (<i>amiA</i>, <i>cgt</i>, <i>cpn60</i>, <i>cpn70</i>, <i>dnaJ</i>, and <i>luxS</i>) related to <i>H. pylori</i> pathogenesis have been performed in order to evaluate their usefulness for the specific detection of <i>H. pylori</i>, the genetic discrimination at strain level and the detection of multiple infection. A total of 52 <i>H. pylori</i> clones, isolated from 14 gastric biopsies from 11 patients, were analyzed for this purpose. <b>Results:</b> All genes were specifically amplified for <i>H. pylori</i> and all clones isolated from different patients were discriminated, with gene distances ranged from 0.9 to 7.8%. Although most clones isolated from the same patient showed identical gene sequences, an event of multiple infection was detected in all the genes and microevolution events were showed for <i>amiA</i> and <i>cpn60</i> genes. <b>Conclusions:</b> These results suggested that housekeeping genes could be useful for <i>H. pylori</i> detection and to elucidate the mode of transmission and the relevance of the multiple infection.
Note: Versió postprint del document publicat a: https://doi.org/10.1111/hel.12304
It is part of: Helicobacter, 2016, vol. 21, p. 481-487
URI: http://hdl.handle.net/2445/168584
Related resource: https://doi.org/10.1111/hel.12304
ISSN: 1083-4389
Appears in Collections:Articles publicats en revistes (Biologia, Sanitat i Medi Ambient)

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