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DC Field | Value | Language |
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dc.contributor.author | Rodríguez Pereira, Cristina | - |
dc.contributor.author | Lagunas, Anna | - |
dc.contributor.author | Casanellas, Ignasi | - |
dc.contributor.author | Vida, Yolanda | - |
dc.contributor.author | Pérez Inestrosa, Ezequiel | - |
dc.contributor.author | Andrades, José A. | - |
dc.contributor.author | Becerra, José | - |
dc.contributor.author | Samitier i Martí, Josep | - |
dc.contributor.author | Blanco, Francisco J. | - |
dc.contributor.author | Magalhaes, Joana | - |
dc.date.accessioned | 2021-03-12T08:45:04Z | - |
dc.date.available | 2021-03-12T08:45:04Z | - |
dc.date.issued | 2020-05-13 | - |
dc.identifier.issn | 1996-1944 | - |
dc.identifier.uri | http://hdl.handle.net/2445/174823 | - |
dc.description.abstract | Aiming to address a stable chondrogenesis derived from mesenchymal stromal cells (MSCs) to be applied in cartilage repair strategies at the onset of osteoarthritis (OA), we analyzed the effect of arginine-glycine-aspartate (RGD) density on cell condensation that occurs during the initial phase of chondrogenesis. For this, we seeded MSC-derived from OA and healthy (H) donors in RGD-dendrimer-poly(L-lactic) acid (PLLA) nanopatterned substrates (RGD concentrations of 4 × 10−9, 10−8, 2.5 × 10−8, and 10−2 w/w), during three days and compared to a cell pellet conventional three-dimensional culture system. Molecular gene expression (collagens type-I and II-COL1A1 and COL2A1, tenascin-TNC, sex determining region Y-box9-SOX9, and gap junction protein alpha 1-GJA1) was determined as well as the cell aggregates and pellet size, collagen type-II and connexin 43 proteins synthesis. This study showed that RGD-tailored first generation dendrimer (RGD-Cys-D1) PLLA nanopatterned substrates supported the formation of pre-chondrogenic condensates from OA- and H-derived human bone marrow-MSCs with enhanced chondrogenesis regarding the cell pellet conventional system (presence of collagen type-II and connexin 43, both at the gene and protein level). A RGD-density dependent trend was observed for aggregates size, in concordance with previous studies. Moreover, the nanopatterns' had a higher effect on OA-derived MSC morphology, leading to the formation of bigger and more compact aggregates with improved expression of early chondrogenic markers. | - |
dc.format.extent | 12 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | MDPI | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.3390/ma13102247 | - |
dc.relation.ispartof | Materials, 2020, vol. 13(10), num. 2247 | - |
dc.relation.uri | https://doi.org/10.3390/ma13102247 | - |
dc.rights | cc-by (c) Rodríguez Pereira, Cristina et al., 2020 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es | - |
dc.source | Articles publicats en revistes (Enginyeria Electrònica i Biomèdica) | - |
dc.subject.classification | Artrosi | - |
dc.subject.classification | Condrogènesi | - |
dc.subject.classification | Medicina regenerativa | - |
dc.subject.other | Osteoarthritis | - |
dc.subject.other | Chondrogenesis | - |
dc.subject.other | Regenerative medicine | - |
dc.title | RGD-Dendrimer-Poly(L-lactic) Acid Nanopatterned Substrates for the Early Chondrogenesis of Human Mesenchymal Stromal Cells Derived from Osteoarthritic and Healthy Donors | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 706089 | - |
dc.date.updated | 2021-03-12T08:45:04Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 32414175 | - |
Appears in Collections: | Articles publicats en revistes (Enginyeria Electrònica i Biomèdica) Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC)) |
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File | Description | Size | Format | |
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706089.pdf | 2.09 MB | Adobe PDF | View/Open |
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