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http://hdl.handle.net/2445/177191
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DC Field | Value | Language |
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dc.contributor.author | Garcia Giménez, Sònia. | - |
dc.contributor.author | Crhák Khaitová, Lucie | - |
dc.contributor.author | Kovarik, Ales | - |
dc.date.accessioned | 2021-05-13T06:59:03Z | - |
dc.date.available | 2021-05-13T06:59:03Z | - |
dc.date.issued | 2012-06-20 | - |
dc.identifier.issn | 1471-2229 | - |
dc.identifier.uri | http://hdl.handle.net/2445/177191 | - |
dc.description.abstract | Background In plants, the 5 S rRNA genes usually occur as separate tandems (S-type arrangement) or, less commonly, linked to 35 S rDNA units (L-type). The activity of linked genes remains unknown so far. We studied the homogeneity and expression of 5 S genes in several species from family Asteraceae known to contain linked 35 S-5 S units. Additionally, their methylation status was determined using bisulfite sequencing. Fluorescence in situ hybridization was applied to reveal the sub-nuclear positions of rDNA arrays. Results We found that homogenization of L-type units went to completion in most (4/6) but not all species. Two species contained major L-type and minor S-type units (termed Ls-type). The linked genes dominate 5 S rDNA expression while the separate tandems do not seem to be expressed. Members of tribe Anthemideae evolved functional variants of the polymerase III promoter in which a residing C-box element differs from the canonical angiosperm motif by as much as 30%. On this basis, a more relaxed consensus sequence of a plant C-box: (5'-RGSWTGGGTG-3') is proposed. The 5 S paralogs display heavy DNA methylation similarly as to their unlinked counterparts. FISH revealed the close association of 35 S-5 S arrays with nucleolar periphery indicating that transcription of 5 S genes may occur in this territory. Conclusions We show that the unusual linked arrangement of 5 S genes, occurring in several plant species, is fully compatible with their expression and functionality. This extraordinary 5 S gene dynamics is manifested at different levels, such as variation in intrachromosomal positions, unit structure, epigenetic modification and considerable divergence of regulatory motifs. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | BioMed Central | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.1186/1471-2229-12-95 | - |
dc.relation.ispartof | BMC Plant Biology, 2012, vol. 12, p. 95 | - |
dc.relation.uri | https://doi.org/10.1186/1471-2229-12-95 | - |
dc.rights | (c) Garcia Giménez, Sònia. et al., 2012 | - |
dc.source | Articles publicats en revistes (Biologia, Sanitat i Medi Ambient) | - |
dc.subject.classification | Artemísia | - |
dc.subject.classification | Epigenètica | - |
dc.subject.classification | Filogènia (Botànica) | - |
dc.subject.classification | Citogenètica | - |
dc.subject.other | Artemisia | - |
dc.subject.other | Epigenetics | - |
dc.subject.other | Phylogeny (Botany) | - |
dc.subject.other | Cytogenetics | - |
dc.title | Expression of 5 S rRNA genes linked to 35 S rDNA in plants, their epigenetic modification and regulatory element divergence | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 647090 | - |
dc.date.updated | 2021-05-13T06:59:03Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 22716941 | - |
Appears in Collections: | Articles publicats en revistes (Biologia, Sanitat i Medi Ambient) |
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File | Description | Size | Format | |
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647090.pdf | 2.32 MB | Adobe PDF | View/Open |
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