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http://hdl.handle.net/2445/179335
Title: | Urinary Cytokines Reflect Renal Inflammation in Acute Tubulointerstitial Nephritis: A Multiplex Bead-Based Assay Assessment |
Author: | Martinez Valenzuela, Laura Draibe, Juliana Bestard, Oriol Fulladosa, Xavier Gómez Preciado, Francisco Antón, Paula Nadal, Ernest Jové, Maria Cruzado, Josep Maria Torras, Juan |
Keywords: | Malalties del ronyó Marcadors bioquímics Citoquines Kidney diseases Biochemical markers |
Issue Date: | 4-Jul-2021 |
Publisher: | MDPI AG |
Abstract: | Background: Acute tubulointerstitial nephritis (ATIN) diagnosis lays on histological assessment through a kidney biopsy, given the absence of accurate non-invasive biomarkers. The aim of this study was to evaluate the accuracy of different urinary inflammation-related cytokines for the diagnostic of ATIN and its distinction from acute tubular necrosis (ATN). Methods: We included 33 patients (ATIN (n = 21), ATN (n = 12)), and 6 healthy controls (HC). We determined the urinary levels of 10 inflammation-related cytokines using a multiplex bead-based Luminex assay at the time of biopsy and after therapy, and registered main clinical, analytical and histological data. Results: At the time of biopsy, urinary levels of I-TAC/CXCL11, CXCL10, IL-6, TNF alpha and MCP-1 were significantly higher in ATIN compared to HC. A positive correlation between the extent of the tubulointerstitial cellular infiltrates in kidney biopsies and the urinary concentration of I-TAC/CXCL11, MIG/CXCL9, CXCL10, IL17, IFN alpha, MCP1 and EGF was observed. Notably, I-TAC/CXCL11, IL-6 and MCP-1 were significantly higher in ATIN than in ATN, with I-TAC/CXCL11 as the best discriminative classifier AUC (0.77, 95% CI 0.57-0.95, p = 0.02). A combinatory model of these three urinary cytokines increased the accuracy in the distinction of ATIN/ATN compared to the individual biomarkers. The best model resulted when combining the three cytokines with blood eosinophil and urinary leukocyte counts (LR = 9.76). Follow-up samples from 11ATIN patients showed a significant decrease in I-TAC/CXCL11, MIG/CXCL9 and CXCL10 levels. Conclusions: Urinary I-TAC/CXCL11, CXCL10, IL6 and MCP-1 levels accurately distinguish patients developing ATIN from ATN and healthy individuals and may serve as novel non-invasive biomarkers in this disease. |
Note: | Reproducció del document publicat a: https://doi.org/10.3390/jcm10132986 |
It is part of: | Journal of Clinical Medicine, 2021, vol. 10, num. 13, p. 2986 |
URI: | http://hdl.handle.net/2445/179335 |
Related resource: | https://doi.org/10.3390/jcm10132986 |
ISSN: | 2077-0383 |
Appears in Collections: | Articles publicats en revistes (Ciències Clíniques) Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
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jcm-10-02986.pdf | 1.44 MB | Adobe PDF | View/Open |
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