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Title: Polymerase-catalyzed synthesis of DNA from phosphoramidate conjugates of deoxynucleotides and amino acids
Author: Adelfinskaya, O.
Terrazas Martínez, Montserrat
Froeyen, M.
Marlière, P.
Nauwelaerts, K.
Herdewijn, P.
Keywords: ADN
Amino acids
Issue Date: 25-Jul-2007
Publisher: Oxford University Press
Abstract: Some selected amino acids, in particular L-aspartic acid (L-Asp) and L-histidine (L-His), can function as leaving group during polymerase-catalyzed incorporation of deoxyadenosine monophosphate (dAMP) in DNA. Although L-Asp-dAMP and L-His-dAMP bind, most probably, in a different way in the active site of the enzyme, aspartic acid and histidine can be considered as mimics of the pyrophosphate moiety of deoxyadenosine triphosphate. L-Aspartic acid is more efficient than D-aspartic acid as leaving group. Such P-N conjugates of amino acids and deoxynucleotides provide a novel experimental ground for diversifying nucleic acid metabolism in the field of synthetic biology.
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It is part of: Nucleic Acids Research, 2007, vol. 35, num. 15, p. 5060-5072
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ISSN: 0305-1048
Appears in Collections:Articles publicats en revistes (Química Inorgànica i Orgànica)

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