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http://hdl.handle.net/2445/179785
Title: | Synthesis and properties of small interfering RNA duplexes carrying 5-ethyluridine residues |
Author: | Terrazas Martínez, Montserrat Eritja i Casadellà, Ramon |
Keywords: | RNA Oligonucleòtids Química orgànica RNA Oligonucleotides Organic chemistry |
Issue Date: | 16-Nov-2010 |
Publisher: | Springer Verlag |
Abstract: | Oligoribonucleotides carrying 5-ethyluridine units were prepared using solid-phase phosphoramidite chemistry. The introduction of the tert-butyldimethylsilyl group at the 2'-OH position proceeded in good yield and very high 2'-regioselectivity. RNA duplexes carrying 5-ethyluridine either at the sense or the guide strands display RNAi activity comparable to or slightly better than that of unmodified RNA duplexes. Gene suppression experiments using luciferase targets in SH-SY5Y cells show that the ethyl group is generally well accepted at all positions although a small decrease in RNA interference activity is observed when one 5-ethylU residue is incorporated in the 3' overhangs. |
Note: | Versió postprint del document publicat a: https://doi.org/10.1039/c003221b |
It is part of: | Molecular Diversity, 2010, vol. 15, num. 3, p. 677-686 |
URI: | http://hdl.handle.net/2445/179785 |
Related resource: | https://doi.org/10.1039/c003221b |
ISSN: | 1381-1991 |
Appears in Collections: | Articles publicats en revistes (Química Inorgànica i Orgànica) Articles publicats en revistes (Institut de Recerca Biomèdica (IRB Barcelona)) |
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