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http://hdl.handle.net/2445/186967
Title: | Comprehensive assessment of myocardial remodeling in ischemic heart disease by synchrotron propagation based X-ray phase contrast imaging. |
Author: | Planinc, Ivo Garcia Canadilla, Patricia Dejea, Hector Ilic, Ivana Guasch i Casany, Eduard Zamora, Monica Crispi Brillas, Fàtima Stampanoni, Marco Milicic, Davor Bijnens, Bart Bonnin, Anne Cikes, Maja |
Keywords: | Diagnòstic per la imatge Raigs X Animals Diagnostic imaging X-rays Animals |
Issue Date: | 7-Jan-2021 |
Publisher: | Nature Publishing Group |
Abstract: | Cardiovascular research is in an ongoing quest for a superior imaging method to integrate grossanatomical information with microanatomy, combined with quantifable parameters of cardiac structure. In recent years, synchrotron radiation-based X-ray Phase Contrast Imaging (X-PCI) has been extensively used to characterize soft tissue in detail. The objective was to use X-PCI to comprehensively quantify ischemic remodeling of diferent myocardial structures, from cell to organ level, in a rat model of myocardial infarction. Myocardial infarction-induced remodeling was recreated in a well-established rodent model. Ex vivo rodent hearts were imaged by propagation based X-PCI using two confgurations resulting in 5.8 µm and 0.65 µm efective pixel size images. The acquired datasets were used for a comprehensive assessment of macrostructural changes including the whole heart and vascular tree morphology, and quantifcation of left ventricular myocardial thickness, mass, volume, and organization. On the meso-scale, tissue characteristics were explored and compared with histopathological methods, while microstructural changes were quantifed by segmentation of cardiomyocytes and calculation of cross-sectional areas. Propagation based X-PCI provides detailed visualization and quantifcation of morphological changes on whole organ, tissue, vascular as well as individual cellular level of the ex vivo heart, with a single, non-destructive 3D imaging modality. |
Note: | Reproducció del document publicat a: https://doi.org/10.1038/s41598-021-93054-6 |
It is part of: | Scientific Reports, 2021, vol. 11, p. 14020 |
URI: | http://hdl.handle.net/2445/186967 |
Related resource: | https://doi.org/10.1038/s41598-021-93054-6 |
ISSN: | 2045-2322 |
Appears in Collections: | Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer) Articles publicats en revistes (Medicina) |
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