Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/191039
Title: | Green Tea catechins modulate skeletal development with effects dependent on dose, time, and structure in a Down syndrome mouse model |
Author: | Llambrich, Sergi González-Colom, Rubèn Wouters, Jens Roldan, Jorge Salassa, Sara Wouters, Kaat Van Bulck, Vicky Sharpe, James Callaerts-Vegh, Zsuzsanna Vande Velde, Greetje Martínez Abadías, Neus, 1978- |
Keywords: | Síndrome de Down Te Antioxidants Down syndrome Tea Antioxidants |
Issue Date: | 7-Oct-2022 |
Publisher: | MDPI |
Abstract: | Altered skeletal development in Down syndrome (DS) results in a brachycephalic skull, flattened face, shorter mandibular ramus, shorter limbs, and reduced bone mineral density (BMD). Our previous study showed that low doses of green tea extract enriched in epigallocatechin-3-gallate (GTE-EGCG), administered continuously from embryonic day 9 to postnatal day 29, reduced facial dysmorphologies in the Ts65Dn (TS) mouse model of DS, but high doses could exacerbate them. Here, we extended the analyses to other skeletal structures and systematically evaluated the effects of high and low doses of GTE-EGCG treatment over postnatal development in wild-type (WT) and TS mice using in vivo µCT and geometric morphometrics. TS mice developed shorter and wider faces, skulls, and mandibles, together with shorter and narrower humerus and scapula, and reduced BMD dynamically over time. Besides facial morphology, GTE-EGCG did not rescue any other skeletal phenotype in TS treated mice. In WT mice, GTE-EGCG significantly altered the shape of the skull and mandible, reduced the length and width of the long bones, and lowered the BMD. The disparate effects of GTE-EGCG depended on the dose, developmental timepoint, and anatomical structure analyzed, emphasizing the complex nature of DS and the need to further investigate the simultaneous effects of GTE-EGCG supplementation. |
Note: | Reproducció del document publicat a: https://doi.org/10.3390/nu14194167 |
It is part of: | Nutrients, 2022, vol. 14, num. 19, p. 4167 |
URI: | http://hdl.handle.net/2445/191039 |
Related resource: | https://doi.org/10.3390/nu14194167 |
ISSN: | 2072-6643 |
Appears in Collections: | Articles publicats en revistes (Biologia Evolutiva, Ecologia i Ciències Ambientals) |
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