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http://hdl.handle.net/2445/201162
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DC Field | Value | Language |
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dc.contributor.author | Soler Vázquez, M. Carmen | - |
dc.contributor.author | Romero Romero, María del Mar | - |
dc.contributor.author | Todorčević, Marijana | - |
dc.contributor.author | Delgado, Katia | - |
dc.contributor.author | Calatayud Aristoy, Carles | - |
dc.contributor.author | Benítez Amaro, Aleyda | - |
dc.contributor.author | La Chica Lhoëst, Maria Teresa | - |
dc.contributor.author | Mera Nanín, Paula | - |
dc.contributor.author | Zagmutt Caroxa, Sebastián | - |
dc.contributor.author | Bastías-Pérez, Marianela | - |
dc.contributor.author | Ibeas, Kevin | - |
dc.contributor.author | Casals, Núria | - |
dc.contributor.author | Escolà Gil, Joan Carles | - |
dc.contributor.author | Llorente Cortés, Vicenta | - |
dc.contributor.author | Consiglio, Antonella | - |
dc.contributor.author | Serra i Cucurull, Dolors | - |
dc.contributor.author | Herrero Rodríguez, Laura | - |
dc.date.accessioned | 2023-07-25T10:12:20Z | - |
dc.date.available | 2023-07-25T10:12:20Z | - |
dc.date.issued | 2023-05 | - |
dc.identifier.issn | 1096-7176 | - |
dc.identifier.other | 37088334 | - |
dc.identifier.uri | http://hdl.handle.net/2445/201162 | - |
dc.description.abstract | Obesity and its associated metabolic comorbidities are a rising global health and social issue, with novel therapeutic approaches urgently needed. Adipose tissue plays a key role in the regulation of energy balance and adipose tissue-derived mesenchymal stem cells (AT-MSCs) have gained great interest in cell therapy. Carnitine palmitoyltransferase 1A (CPT1A) is the gatekeeper enzyme for mitochondrial fatty acid oxidation. Here, we aimed to generate adipocytes expressing a constitutively active CPT1A form (CPT1AM) that can improve the obese phenotype in mice after their implantation. AT-MSCs were differentiated into mature adipocytes, subjected to lentivirus-mediated expression of CPT1AM or the GFP control, and subcutaneously implanted into mice fed a high-fat diet (HFD). CPT1AM-implanted mice showed lower body weight, hepatic steatosis and serum insulin and cholesterol levels alongside improved glucose tolerance. HFD-induced increases in adipose tissue hypertrophy, fibrosis, inflammation, endoplasmic reticulum stress and apoptosis were reduced in CPT1AM-implanted mice. In addition, the expression of mitochondrial respiratory chain complexes was enhanced in the adipose tissue of CPT1AM-implanted mice. Our results demonstrate that implantation of CPT1AM-expressing AT-MSC-derived adipocytes into HFD-fed mice improves the obese metabolic phenotype, supporting the future clinical use of this ex vivo gene therapy approach. | - |
dc.format.extent | 17 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.1016/j.ymben.2023.04.010 | - |
dc.relation.ispartof | Metabolic Engineering, 2023, vol. 77, p. 256-272 | - |
dc.relation.uri | https://doi.org/10.1016/j.ymben.2023.04.010 | - |
dc.rights | cc-by-nc-nd (c) Soler-Vázquez, M. Carmen et al., 2023 | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | - |
dc.source | Articles publicats en revistes (Patologia i Terapèutica Experimental) | - |
dc.subject.classification | Obesitat | - |
dc.subject.classification | Teixit adipós | - |
dc.subject.classification | Inflamació | - |
dc.subject.classification | Glucosa | - |
dc.subject.classification | Ratolins (Animals de laboratori) | - |
dc.subject.other | Obesity | - |
dc.subject.other | Adipose tissues | - |
dc.subject.other | Inflammation | - |
dc.subject.other | Glucose | - |
dc.subject.other | Mice (Laboratory animals) | - |
dc.title | Implantation of CPT1AM-expressing adipocytes reduces obesity and glucose intolerance in mice | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 733485 | - |
dc.date.updated | 2023-07-25T10:12:20Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
Appears in Collections: | Articles publicats en revistes (Bioquímica i Fisiologia) Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) Articles publicats en revistes (Patologia i Terapèutica Experimental) |
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File | Description | Size | Format | |
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733485.pdf | 11.56 MB | Adobe PDF | View/Open |
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