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http://hdl.handle.net/2445/34009
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DC Field | Value | Language |
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dc.contributor.author | Nogueira, Daniele R. | - |
dc.contributor.author | Tavano, Lorena | - |
dc.contributor.author | Mitjans Arnal, Montserrat | - |
dc.contributor.author | Pérez Muñoz, Lourdes | - |
dc.contributor.author | Infante Martínez-Pardo, Ma. Rosa | - |
dc.contributor.author | Vinardell Martínez-Hidalgo, Ma. Pilar | - |
dc.date.accessioned | 2013-02-27T15:39:57Z | - |
dc.date.available | 2013-02-27T15:39:57Z | - |
dc.date.issued | 2013-04 | - |
dc.identifier.issn | 0142-9612 | - |
dc.identifier.uri | http://hdl.handle.net/2445/34009 | - |
dc.description.abstract | Nanoparticles with pH-sensitive behavior may enhance the success of chemotherapy in many cancers by efficient intracellular drug delivery. Here, we investigated the effect of a bioactive surfactant with pH-sensitive properties on the antitumor activity and intracellular behavior of methotrexate-loaded chitosan nanoparticles (MTX-CS-NPs). NPs were prepared using a modified ionotropic complexation process, in which was included the surfactant derived from Nα,Nε-dioctanoyl lysine with an inorganic lithium counterion. The pH-sensitive behavior of NPs allowed accelerated release of MTX in an acidic medium, as well as membrane-lytic pH-dependent activity, which facilitated the cytosolic delivery of endocytosed materials. Moreover, our results clearly proved that MTX-CSNPs were more active against the tumor HeLa and MCF-7 cell lines than the free drug. The feasibilty of using NPs to target acidic tumor extracellular pH was also shown, as cytotoxicity against cancer cells was greater in a mildly acidic environment. Finally, the combined physicochemical and pH-sensitive properties of NPs generally allowed the entrapped drug to induce greater cell cycle arrest and apoptotic effects. Therefore, our overall results suggest that pH-sensitive MTX-CS-NPs could be potentially useful as a carrier system for tumor and intracellular drug delivery in cancer therapy. | - |
dc.format.extent | 15 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier B.V. | - |
dc.relation.isformatof | Versió postprint del document publicat a: http://dx.doi.org/10.1016/j.biomaterials.2013.01.005 | - |
dc.relation.ispartof | Biomaterials, 2013, vol. 34, núm. 11, p. 2758-2772 | - |
dc.relation.uri | http://dx.doi.org/10.1016/j.biomaterials.2013.01.005 | - |
dc.rights | (c) Elsevier B.V., 2013 | - |
dc.source | Articles publicats en revistes (Bioquímica i Fisiologia) | - |
dc.subject.classification | Agents tensioactius | - |
dc.subject.classification | Membranes cel·lulars | - |
dc.subject.classification | Nanopartícules | - |
dc.subject.classification | Citotoxicitat per mediació cel·lular | - |
dc.subject.classification | Nanotoxicologia | - |
dc.subject.other | Surface active agents | - |
dc.subject.other | Cell membranes | - |
dc.subject.other | Nanoparticles | - |
dc.subject.other | Cell-mediated cytotoxicity | - |
dc.subject.other | Nanotoxicology | - |
dc.title | In vitro antitumor activity of methotrexate via pH-sensitive chitosan nanoparticles | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/acceptedVersion | - |
dc.identifier.idgrec | 618932 | - |
dc.date.updated | 2013-02-27T15:39:57Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 23352041 | - |
Appears in Collections: | Articles publicats en revistes (Bioquímica i Fisiologia) |
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File | Description | Size | Format | |
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618932.pdf | 729.09 kB | Adobe PDF | View/Open |
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