Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/34504
Title: A carboxylesterase, Esterase-6, modulates sensory physiological and behavioural response dynamics to pheromone in Drosophila
Author: Chertemps, Thomas
François, Adrien
Durand, Nicolas
Rosell Pellisé, Glòria
Dekker, Teun
Lucas, Philippe
Maïbèche-Coisne, Martine
Keywords: Drosòfila
Enzims
Feromones
Etologia
Olors
Esterases
Drosophila
Enzymes
Pheromones
Animal behavior
Odors
Esterases
Issue Date: 21-Jun-2012
Publisher: BioMed Central
Abstract: Background: Insects respond to the spatial and temporal dynamics of a pheromone plume, which implies not only a strong response to"odor on", but also to"odor off". This requires mechanisms geared toward a fast signal termination. Several mechanisms may contribute to signal termination, among which odorant-degrading enzymes. These enzymes putatively play a role in signal dynamics by a rapid inactivation of odorants in the vicinity of the sensory receptors, although direct in vivo experimental evidences are lacking. Here we verified the role of an extracellular carboxylesterase, esterase-6 (Est-6), in the sensory physiological and behavioral dynamics of Drosophila melanogaster response to its pheromone, cis-vaccenyl acetate (cVA). Est-6 was previously linked to post-mating effects in the reproductive system of females. As Est-6 is also known to hydrolyze cVA in vitro and is expressed in the main olfactory organ, the antenna, we tested here its role in olfaction as a putative odorant-degrading enzyme. Results: We first confirm that Est-6 is highly expressed in olfactory sensilla, including cVA-sensitive sensilla, and we show that expression is likely associated with non-neuronal cells. Our electrophysiological approaches show that the dynamics of olfactory receptor neuron (ORN) responses is strongly influenced by Est-6, as in Est-6° null mutants (lacking the Est-6 gene) cVA-sensitive ORN showed increased firing rate and prolonged activity in response to cVA. Est-6° mutant males had a lower threshold of behavioral response to cVA, as revealed by the analysis of two cVAinduced behaviors. In particular, mutant males exhibited a strong decrease of male-male courtship, in association with a delay in courtship initiation. Conclusions: Our study presents evidence that Est-6 plays a role in the physiological and behavioral dynamics of sex pheromone response in Drosophila males and supports a role of Est-6 as an odorant-degrading enzyme (ODE) in male antennae. Our results also expand the role of Est-6 in Drosophila biology, from reproduction to olfaction, and highlight the role of ODEs in insect olfaction. Keywords: carboxylesterase, esterase 6, olfaction, pheromone, signal termination
Note: Reproducció del document publicat a: http://dx.doi.org/10.1186/1741-7007-10-56
It is part of: Bmc Biology, 2012, vol. 10, num. 56
Related resource: http://dx.doi.org/10.1186/1741-7007-10-56
URI: http://hdl.handle.net/2445/34504
ISSN: 1741-7007
Appears in Collections:Articles publicats en revistes (Farmacologia, Toxicologia i Química Terapèutica)

Files in This Item:
File Description SizeFormat 
590008.pdf714.29 kBAdobe PDFView/Open


This item is licensed under a Creative Commons License Creative Commons