Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/42597
Title: Experimental Identification of Actinobacillus pleuropneumoniae Strains L20 and JL03 Heptosyltransferases, Evidence for a New Heptosyltransferase Signature Sequence.
Author: Merino Montero, Susana
Knirel, Yuriy A.
Regué Queralt, Miguel, 1953-
Tomàs Magaña, Juan
Keywords: Bacteris
Genètica bacteriana
Antígens
Bacteria
Bacterial genetics
Antigens
Issue Date: 2013
Publisher: Public Library of Science (PLoS)
Abstract: We experimentally identified the activities of six predicted heptosyltransferases in Actinobacillus pleuropneumoniae genome serotype 5b strain L20 and serotype 3 strain JL03. The initial identification was based on a bioinformatic analysis of the amino acid similarity between these putative heptosyltrasferases with others of known function from enteric bacteria and Aeromonas. The putative functions of all the Actinobacillus pleuropneumoniae heptosyltrasferases were determined by using surrogate LPS acceptor molecules from well-defined A. hydrophyla AH-3 and A. salmonicida A450 mutants. Our results show that heptosyltransferases APL_0981 and APJL_1001 are responsible for the transfer of the terminal outer core D-glycero-D-manno-heptose (D,D-Hep) residue although they are not currently included in the CAZY glycosyltransferase 9 family. The WahF heptosyltransferase group signature sequence [S(T/S)(GA)XXH] differs from the heptosyltransferases consensus signature sequence [D(TS)(GA)XXH], because of the substitution of D(261) for S(261), being unique.
Note: Reproducció del document publicat a: http://dx.doi.org/10.1371/journal.pone.0055546
It is part of: PLoS One, 2013, vol. 8, num. 1, p. e55546-e55546
Related resource: http://dx.doi.org/10.1371/journal.pone.0055546
URI: http://hdl.handle.net/2445/42597
ISSN: 1932-6203
Appears in Collections:Articles publicats en revistes (Biologia, Sanitat i Medi Ambient)
Articles publicats en revistes (Genètica, Microbiologia i Estadística)

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