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Title: Structure and Physicochemical Properties of Phospholipidic Monolayers and Bilayers. LB and AFM Studies
Author: Domènech Cabrera, Òscar
Director: Sanz Carrasco, Fausto
Borrell Hernández, Jordi
Keywords: Mitocòndries
Issue Date: 27-Feb-2007
Publisher: Universitat de Barcelona
Abstract: [eng] The main objective of this Ph. D. Thesis work was to study the physicochemical properties of the inner membrane of mitochondria and the interaction of "cyt c" with model membranes. Suitable techniques were used to accomplish this objective: Langmuir and Langmuir Blodgett films, fluorescence spectroscopy, Brewster Angle Microscopy and Atomic Force Microscopy. The general conclusion of this Thesis is: "Calcium induces HII phases in POPE:CL (0.8:0.2, mol:mol) samples in solution. This composition is the most stable mixed monolayer of both phospholipids and corresponds to the native molar fraction in the inner membrane of the mitochondrion. The extension of these inverted micelles forms planar bilayers on solid supports displaying lateral lipid segregation of CL enriched domains where cyt "c can" bind specifically. In this configuration POPC can remain as a spacer in POPE enriched domains forming the matrix of the membrane". The inverse process would be a possible vindication to the release of "cyt c" from the inner membrane of mitochondrion during apoptosis.
ISBN: 9788469163719
Appears in Collections:Tesis Doctorals - Departament - Química Física

Files in This Item:
File Description SizeFormat 
00.ODC_Introduction.pdf1.1 MBAdobe PDFView/Open
01.ODC_Objectives_Methods.pdf801.11 kBAdobe PDFView/Open
02.ODC_Results.pdf2.96 MBAdobe PDFView/Open
03.ODC_Discussion.pdf359.26 kBAdobe PDFView/Open
04.ODC_Conclusions_References.pdf238.65 kBAdobe PDFView/Open
05.ODC_Appendix_I.pdf96.76 kBAdobe PDFView/Open
06.ODC_Appendix_II.pdf6.12 MBAdobe PDFView/Open
07.ODC_Appendix_III.pdf1.31 MBAdobe PDFView/Open

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