Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/43593
Title: Cyanobacterial diversity and a new Acaryochloris-like symbiont from Bahamian sea-squirts
Author: López Legentil, Susanna
Song, B.
Bosch Marimon, Manel
Pawlik, J.R.
Turon Barrera, Xavier
Keywords: Invertebrats marins
Cianobacteris
Ecologia marina
Ascidiacis
Bahames
Marine invertebrates
Cyanobacteria
Marine ecology
Sea squirts
Bahamas
Issue Date: Aug-2011
Publisher: Public Library of Science (PLoS)
Abstract: Symbiotic interactions between ascidians (sea-squirts) and microbes are poorly understood. Here we characterized the cyanobacteria in the tissues of 8 distinct didemnid taxa from shallow-water marine habitats in the Bahamas Islands by sequencing a fragment of the cyanobacterial 16S rRNA gene and the entire 16S-23S rRNA internal transcribed spacer region (ITS) and by examining symbiont morphology with transmission electron (TEM) and confocal microscopy (CM). As described previously for other species, Trididemnum spp. mostly contained symbionts associated with the Prochloron-Synechocystis group. However, sequence analysis of the symbionts in Lissoclinum revealed two unique clades. The first contained a novel cyanobacterial clade, while the second clade was closely associated with Acaryochloris marina. CM revealed the presence of chlorophyll d (chl d) and phycobiliproteins (PBPs) within these symbiont cells, as is characteristic of Acaryochloris species. The presence of symbionts was also observed by TEM inside the tunic of both the adult and larvae of L. fragile, indicating vertical transmission to progeny. Based on molecular phylogenetic and microscopic analyses, Candidatus Acaryochloris bahamiensis nov. sp. is proposed for this symbiotic cyanobacterium. Our results support the hypothesis that photosymbiont communities in ascidians are structured by host phylogeny, but in some cases, also by sampling location.
Note: Reproducció del document publicat a: http://dx.doi.org/10.1371/journal.pone.0023938
It is part of: PLoS One, 2011, vol. 6, num. 8, p. e23938
Related resource: http://dx.doi.org/10.1371/journal.pone.0023938
URI: http://hdl.handle.net/2445/43593
ISSN: 1932-6203
Appears in Collections:Articles publicats en revistes (Biologia Evolutiva, Ecologia i Ciències Ambientals)

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