Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/43778
Title: The embryonic blood-CSF barrier has molecular elements for specific glucose transport and for the general transport of molecules via transcellular routes.
Author: Parvas, Maryam
Bueno i Torrens, David, 1965-
Keywords: Cervell
Sistema nerviós central
Neurobiologia del desenvolupament
Brain
Central nervous system
Developmental neurobiology
Issue Date: 2010
Publisher: Scientific Research Publishing
Abstract: In vertebrates, early brain development takes place at the expanded anterior end of the neural tube, which is filled with embryonic cerebrospinal fluid (E-CSF). We have recently identified a transient blood-CSF barrier that forms between embryonic days E3 and E4 in chick embryos and that is responsible for the transport of proteins and control of E-CSF homeostasis, including osmolarity. Here we examined the presence of glucose transporter GLUT-1 as well the presence of caveolae-structural protein Caveolin1 (CAV-1) in the embryonic blood-CSF barrier which may be involved in the transport of glucose and of proteins, water and ions respectively across the neuroectoderm. In this paper we demonstrate the presence of GLUT-1 and CAV-1 in endothelial cells of blood vessels as well as in adjacent neuroectodermal cells, located in the embryonic blood-CSF barrier. In blood vessels, these proteins were detected as early as E4 in chick embryos and E12.7 in rat embryos, i.e. the point at which the embryonic blood-CSF barrier acquires this function. In the neuroectoderm of the embryonic blood-CSF barrier, GLUT-1 was also detected at E4 and E12.7 respectively, and CAV-1 was detected shortly thereafter in both experimental models. These experiments contribute to delineating the extent to which the blood-CSF embryonic barrier controls E-CSF composition and homeostasis during early stages of brain development in avians and mammals. Our results suggest the regulation of glucose transport to the E-CSF by means of GLUT-1 and also suggest a mechanism by which proteins are transported via transcellular routes across the neuroectoderm, thus reinforcing the crucial role of E-CSF in brain development.
Note: Reproducció del document publicat a: http://dx.doi.org/10.4236/abb.2010.14041
It is part of: Advances in Bioscience and Biotechnology , 2010, vol. 1, p. 315-321
Related resource: http://dx.doi.org/10.4236/abb.2010.14041
URI: http://hdl.handle.net/2445/43778
ISSN: 2156-8456
Appears in Collections:Articles publicats en revistes (Genètica, Microbiologia i Estadística)

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