Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/44155
Title: Allelic diversity and population structure in Vibrio cholerae O139 Bengal based on nucleotide sequence analysis
Author: Farfán Sellarés, Maribel
Miñana i Galbis, David
Fusté Munné, M. Carme
Lorén Egea, José Gaspar
Keywords: Epidemiologia
Genètica bacteriana
ADN
Recombinació genètica
Còlera
Epidemiology
Bacterial genetics
DNA
Genetic recombination
Cholera
Issue Date: Mar-2002
Publisher: American Society for Microbiology
Abstract: Comparative analysis of gene fragments of six housekeeping loci, distributed around the two chromosomes of Vibrio cholerae, has been carried out for a collection of 29 V. cholerae O139 Bengal strains isolated from India during the first epidemic period (1992 to 1993). A toxigenic O1 ElTor strain from the seventh pandemic and an environmental non-O1/non-O139 strain were also included in this study. All loci studied were polymorphic, with a small number of polymorphic sites in the sequenced fragments. The genetic diversity determined for our O139 population is concordant with a previous multilocus enzyme electrophoresis study in which we analyzed the same V. cholerae O139 strains. In both studies we have found a higher genetic diversity than reported previously in other molecular studies. The results of the present work showed that O139 strains clustered in several lineages of the dendrogram generated from the matrix of allelic mismatches between the different genotypes, a finding which does not support the hypothesis previously reported that the O139 serogroup is a unique clone. The statistical analysis performed in the V. cholerae O139 isolates suggested a clonal population structure. Moreover, the application of the Sawyer's test and split decomposition to detect intragenic recombination in the sequenced gene fragments did not indicate the existence of recombination in our O139 population.
Note: Reproducció del document publicat a: http://dx.doi.org/10.1128/JB.184.5.1304-1313.2002
It is part of: Journal of Bacteriology, 2002, vol. 184, num. 5, p. 1304-1313
Related resource: http://dx.doi.org/10.1128/JB.184.5.1304-1313.2002
URI: http://hdl.handle.net/2445/44155
ISSN: 0021-9193
Appears in Collections:Articles publicats en revistes (Biologia, Sanitat i Medi Ambient)

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