Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/52923
Title: Biosynthesis of panaxynol and panaxydol in Panax ginseng
Author: Knispel, Nihat
Ostrozhenkova, Elena
Schramek, Nicholas
Huber, Claudia
Peña-Rodríguez, Luis M.
Bonfill Baldrich, Ma. Mercedes
Palazón Barandela, Javier
Wischmann, Gesine
Cusidó Vidal, Rosa M.
Eisenreich, Wolfgang
Keywords: Biosíntesi
Ginseng
Biosynthesis
Ginseng
Issue Date: 2-Jul-2013
Publisher: MDPI Publishing
Abstract: The natural formation of the bioactive C17-polyacetylenes (−)-(R)-panaxynol and panaxydol was analyzed by 13C-labeling experiments. For this purpose, plants of Panax ginseng were supplied with 13CO2 under field conditions or, alternatively, sterile root cultures of P. ginseng were supplemented with [U-13C6]glucose. The polyynes were isolated from the labeled roots or hairy root cultures, respectively, and analyzed by quantitative NMR spectroscopy. The same mixtures of eight doubly 13C-labeled isotopologues and one single labeled isotopologue were observed in the C17-polyacetylenes obtained from the two experiments. The polyketide-type labeling pattern is in line with the biosynthetic origin of the compounds via decarboxylation of fatty acids, probably of crepenynic acid. The 13C-study now provides experimental evidence for the biosynthesis of panaxynol and related polyacetylenes in P. ginseng under in planta conditions as well as in root cultures. The data also show that 13CO2 experiments under field conditions are useful to elucidate the biosynthetic pathways of metabolites, including those from roots.
Note: Reproducció del document publicat a: http://dx.doi.org/10.3390/molecules18077686
It is part of: Molecules, 2013, vol. 18, p. 7686-7698
Related resource: http://dx.doi.org/10.3390/molecules18077686
URI: http://hdl.handle.net/2445/52923
ISSN: 1420-3049
Appears in Collections:Articles publicats en revistes (Biologia, Sanitat i Medi Ambient)

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