Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/53114
Title: Modulation in Wistar rats of blood corticosterone compartmentation by sex and prior exposure to a cafeteria diet
Author: Romero Romero, María del Mar
Holmgren-Holm, Fredrik
Grasa Martínez, Maria del Mar
Esteve Ràfols, Montserrat
Remesar Betlloch, Xavier
Fernández López, José Antonio
Alemany, Marià, 1946-
Keywords: Dieta
Hormones esteroides
Corticosteroides
Diferències entre sexes
Rates (Animals de laboratori)
Diet
Steroid hormones
Adrenocortical hormones
Sex differences
Rats as laboratory animals
Issue Date: 22-Feb-2013
Publisher: Public Library of Science (PLoS)
Abstract: In the metabolic syndrome, glucocorticoid activity is increased, but circulating levels show little change. Most of blood glucocorticoids are bound to corticosteroid-binding globulin (CBG), which liver expression and circulating levels are higher in females than in males. Since blood hormones are also bound to blood cells, and the size of this compartment is considerable for androgens and estrogens, we analyzed whether sex or eating a cafeteria diet altered the compartmentation of corticosterone in rat blood. The main corticosterone compartment in rat blood is that specifically bound to plasma proteins, with smaller compartments bound to blood cells or free. Cafeteria diet increased the expression of liver CBG gene, binding plasma capacity and the proportion of blood cell-bound corticosterone. There were marked sex differences in blood corticosterone compartmentation in rats, which were unrelated to testosterone. The use of a monoclonal antibody ELISA and a polyclonal Western blot for plasma CBG compared with both specific plasma binding of corticosterone and CBG gene expression suggested the existence of different forms of CBG, with varying affinities for corticosterone in males and females, since ELISA data showed higher plasma CBG for males, but binding and Western blot analyses (plus liver gene expression) and higher physiological effectiveness for females. Good cross- reactivity to the antigen for polyclonal CBG antibody suggests that in all cases we were measuring CBG.The different immunoreactivity and binding affinity may help explain the marked sex-related differences in plasma hormone binding as sex-linked different proportions of CBG forms.
Note: Reproducció del document publicat a: http://dx.doi.org/10.1371/journal.pone.0057342
It is part of: PLoS One, 2013, vol. 8, num. 2, p. e57342
Related resource: http://dx.doi.org/10.1371/journal.pone.0057342
URI: http://hdl.handle.net/2445/53114
ISSN: 1932-6203
Appears in Collections:Articles publicats en revistes (Nutrició, Ciències de l'Alimentació i Gastronomia)

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