Please use this identifier to cite or link to this item:
Title: Substrate-selective repair and restart of replication forks by DNA translocases
Author: Bétous, Rémy
Couch, Frank B.
Mason, Aaron C.
Eichman, Brandt F.
Mañosas Castejón, María
Cortez, David
Keywords: ADN
Cèl·lules canceroses
Escheríchia coli
Reparació de l'ADN
Cancer cells
Escherichia coli
DNA repair
Issue Date: 27-Jun-2013
Publisher: Elsevier
Abstract: Stalled replication forks are sources of genetic instability. Multiple fork-remodeling enzymes are recruited to stalled forks, but how they work to promote fork restart is poorly understood. By combining ensemble biochemical assays and single-molecule studies with magnetic tweezers, we show that SMARCAL1 branch migration and DNA-annealing activities are directed by the single-stranded DNA-binding protein RPA to selectively regress stalled replication forks caused by blockage to the leading-strand polymerase and to restore normal replication forks with a lagging-strand gap. We unveil the molecular mechanisms by which RPA enforces SMARCAL1 substrate preference. E. coli RecG acts similarly to SMARCAL1 in the presence of E. coli SSB, whereas the highly related human protein ZRANB3 has different substrate preferences. Our findings identify the important substrates of SMARCAL1 in fork repair, suggest that RecG and SMARCAL1 are functional orthologs, and provide a comprehensive model of fork repair by these DNA translocases.
Note: Reproducció del document publicat a:
It is part of: Cell Reports, 2013, vol. 3, num. 6, p. 1958-1969
Related resource:
ISSN: 2211-1247
Appears in Collections:Articles publicats en revistes (Física de la Matèria Condensada)

Files in This Item:
File Description SizeFormat 
631797.pdf2.6 MBAdobe PDFView/Open

This item is licensed under a Creative Commons License Creative Commons