Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/53323
Title: | Holographic optical tweezers combined with back-focal-plane displacement detection |
Author: | Marsà, Ferran Farré Flaquer, Arnau Martín Badosa, Estela Montes Usategui, Mario |
Keywords: | Nanotecnologia Holografia ADN Aparells i instruments científics Biofísica Nanotechnology Holography DNA Scientific apparatus and instruments Biophysics |
Issue Date: | 16-Dec-2013 |
Publisher: | Optical Society of America |
Abstract: | A major problem with holographic optical tweezers (HOTs) is their incompatibility with laser-based position detection methods, such as back-focal-plane interferometry (BFPI). The alternatives generally used with HOTs, like high-speed video tracking, do not offer the same spatial and temporal bandwidths. This has limited the use of this technique in precise quantitative experiments. In this paper, we present an optical trap design that combines digital holography and back-focal-plane displacement detection. We show that, with a particularly simple setup, it is possible to generate a set of multiple holographic traps and an additional static non-holographic trap with orthogonal polarizations and that they can be, therefore, easily separated for measuring positions and forces with the high positional and temporal resolutions of laser-based detection. We prove that measurements from both polarizations contain less than 1% crosstalk and that traps in our setup are harmonic within the typical range. We further tested the instrument in a DNA stretching experiment and we discuss an interesting property of this configuration: the small drift of the differential signal between traps. |
Note: | Reproducció del document publicat a: http://dx.doi.org./10.1364/OE.21.030282 |
It is part of: | Optics Express, 2013, vol. 21, num. 25, p. 30282-30294 |
URI: | http://hdl.handle.net/2445/53323 |
Related resource: | http://dx.doi.org/10.1364/OE.21.030282 |
ISSN: | 1094-4087 |
Appears in Collections: | Articles publicats en revistes (Física Aplicada) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
630821.pdf | 5.99 MB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.