Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/53323
Title: Holographic optical tweezers combined with back-focal-plane displacement detection
Author: Marsà, Ferran
Farré, Arnau
Martín Badosa, Estela
Montes Usategui, Mario
Keywords: Nanotecnologia
Holografia
ADN
Aparells i instruments científics
Biofísica
Nanotechnology
Holography
DNA
Scientific apparatus and instruments
Biophysics
Issue Date: 16-Dec-2013
Publisher: Optical Society of America
Abstract: A major problem with holographic optical tweezers (HOTs) is their incompatibility with laser-based position detection methods, such as back-focal-plane interferometry (BFPI). The alternatives generally used with HOTs, like high-speed video tracking, do not offer the same spatial and temporal bandwidths. This has limited the use of this technique in precise quantitative experiments. In this paper, we present an optical trap design that combines digital holography and back-focal-plane displacement detection. We show that, with a particularly simple setup, it is possible to generate a set of multiple holographic traps and an additional static non-holographic trap with orthogonal polarizations and that they can be, therefore, easily separated for measuring positions and forces with the high positional and temporal resolutions of laser-based detection. We prove that measurements from both polarizations contain less than 1% crosstalk and that traps in our setup are harmonic within the typical range. We further tested the instrument in a DNA stretching experiment and we discuss an interesting property of this configuration: the small drift of the differential signal between traps.
Note: Reproducció del document publicat a: http://dx.doi.org./10.1364/OE.21.030282
It is part of: Optics Express, 2013, vol. 21, num. 25, p. 30282-30294
Related resource: http://dx.doi.org/10.1364/OE.21.030282
URI: http://hdl.handle.net/2445/53323
ISSN: 1094-4087
Appears in Collections:Articles publicats en revistes (Física Aplicada)

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