Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/53665
Title: Passive experimental autoimmune encephalomyelitis in C57BL/6 with MOG: evidence of involvement of B cells
Author: Mannara, F.
Valente, Tony
Saura Martí, Josep
Graus Ribas, Francesc
Saiz Hinajeros, Albert
Moreno, B.
Keywords: Malalties neurodegeneratives
Cèl·lules B
Encefalomielitis
Neurodegenerative Diseases
B cells
Encephalomyelitis
Issue Date: 26-Dec-2012
Publisher: Public Library of Science (PLoS)
Abstract: Experimental autoimmune encephalomyelitis (EAE) is the most relevant animal model to study demyelinating diseases such as multiple sclerosis. EAE can be induced by active (active EAE) or passive (at-EAE) transfer of activated T cells in several species and strains of rodents. However, histological features of at-EAE model in C57BL/6 are poorly described. The aim of this study was to characterize the neuroinflammatory and neurodegenerative responses of at-EAE in C57BL/6 mice by histological techniques and compare them with that observed in the active EAE model. To develop the at-EAE, splenocytes from active EAE female mice were harvested and cultured in presence of MOG 35-55 and IL-12, and then injected intraperitoneally in recipient female C57BL6/J mice. In both models, the development of EAE was similar except for starting before the onset of symptoms and presenting a higher EAE cumulative score in the at-EAE model. Spinal cord histological examination revealed an increased glial activation as well as more extensive demyelinating areas in the at-EAE than in the active EAE model. Although inflammatory infiltrates composed by macrophages and T lymphocytes were found in the spinal cord and brain of both models, B lymphocytes were significantly increased in the at-EAE model. The co-localization of these B cells with IgG and their predominant distribution in areas of demyelination would suggest that IgG-secreting B cells are involved in the neurodegenerative processes associated with at-EAE.
Note: Reproducció del document publicat a: http://dx.doi.org/10.1371/journal.pone.0052361
It is part of: PLoS One, 2012, vol. 7, p. e52361
Related resource: http://dx.doi.org/10.1371/journal.pone.0052361
URI: http://hdl.handle.net/2445/53665
ISSN: 1932-6203
Appears in Collections:Articles publicats en revistes (Ciències Fisiològiques)

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