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Title: Promoter DNA Hypermethylation and Gene Repression in Undifferentiated Arabidopsis Cells
Author: Berdasco, María
Alcázar Hernández, Rubén
García-Ortiz, María Victoria
Ballestar Tarín, Esteban
Fernández, Agustín F.
Roldán-Arjona, Teresa
Fernández Tiburcio, Antonio
Altabella Artigas, Teresa
Buisine, Nicolas
Quesneville, Hadi
Baudry, Antoine
Lepiniec, Loïc
Alaminos, Miguel
Rodríguez, Roberto
Lloyd, Alan
Colot, Vincent
Bender, Judith
Canal, María Jesús
Esteller, Manel
Fraga, Mario F.
Keywords: Arabidopsis
Diferenciació cel·lular
Cell diferentiation
Issue Date: 1-Oct-2008
Publisher: Public Library of Science (PLoS)
Abstract: Maintaining and acquiring the pluripotent cell state in plants is critical to tissue regeneration and vegetative multiplication. Histone-based epigenetic mechanisms are important for regulating this undifferentiated state. Here we report the use of genetic and pharmacological experimental approaches to show that Arabidopsis cell suspensions and calluses specifically repress some genes as a result of promoter DNA hypermethylation. We found that promoters of the MAPK12, GSTU10 and BXL1 genes become hypermethylated in callus cells and that hypermethylation also affects the TTG1, GSTF5, SUVH8, fimbrin and CCD7 genes in cell suspensions. Promoter hypermethylation in undifferentiated cells was associated with histone hypoacetylation and primarily occurred at CpG sites. Accordingly, we found that the process specifically depends on MET1 and DRM2 methyltransferases, as demonstrated with DNA methyltransferase mutants. Our results suggest that promoter DNA methylation may be another important epigenetic mechanism for the establishment and/or maintenance of the undifferentiated state in plant cells.
Note: Reproducció del document publicat a:
It is part of: PLoS One, 2008, vol. 3, num. 10, p. e3306
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ISSN: 1932-6203
Appears in Collections:Articles publicats en revistes (Biologia, Sanitat i Medi Ambient)

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