Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/62005
Title: Oleoyl Coenzyme A regulates interaction of transcriptional regulator RaaS (Rv1219c) with DNA in Mycobacteria
Author: Turapov, O.
Waddell, S. J.
Burke, B.
Glenn, S.
Sarybaeva, A. A.
Tudó i Vilanova, Griselda
Labesse, J.
Young, D. I.
Young, M.
Andrew, P. W.
Butcher, P. D.
Cohen Gonsaun, M.
Mukamolova, G.
Keywords: Mycobacterium tuberculosis
Àcid oleic
Antibiòtics
Mycobacterium tuberculosis
Oleic acid
Antibiotics
Issue Date: 10-Jul-2014
Publisher: American Society for Biochemistry and Molecular Biology
Abstract: We have recently shown that RaaS (regulator of antimicrobial-assisted survival), encoded by Rv1219c in Mycobacterium tuberculosis and by bcg_1279c in Mycobacterium bovis bacillus Calmette-Guérin, plays an important role in mycobacterial survival in prolonged stationary phase and during murine infection. Here, we demonstrate that long chain acyl-CoA derivatives (oleoyl-CoA and, to lesser extent, palmitoyl-CoA) modulate RaaS binding to DNA and expression of the downstream genes that encode ATP-dependent efflux pumps. Moreover, exogenously added oleic acid influences RaaS-mediated mycobacterial improvement of survival and expression of the RaaS regulon. Our data suggest that long chain acyl-CoA derivatives serve as biological indicators of the bacterial metabolic state. Dysregulation of efflux pumps can be used to eliminate non-growing mycobacteria.
Note: Versió postprint del document publicat a: http://dx.doi.org/10.1074/jbc.M114.577338
It is part of: Journal of Biological Chemistry, 2014, vol. 289, num. 36, p. 25241-25249
Related resource: http://dx.doi.org/10.1074/jbc.M114.577338
http://creativecommons.org/licenses/by/3.0/
URI: http://hdl.handle.net/2445/62005
ISSN: 0021-9258
Appears in Collections:Articles publicats en revistes (Fonaments Clínics)

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