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Title: | Oleoyl Coenzyme A regulates interaction of transcriptional regulator RaaS (Rv1219c) with DNA in Mycobacteria |
Author: | Turapov, Obolbek Waddell, Simon J. Burke, Bernard Glenn, Sarah Sarybaeva, Asel A. Tudó i Vilanova, Griselda Labesse, Gilles Young, Danielle I. Young, Michael Andrew, Peter W. Butcher, Philip D. Cohen Gonsaun, Martin Mukamolova, Galina |
Keywords: | Mycobacterium tuberculosis Àcid oleic Antibiòtics Mycobacterium tuberculosis Oleic acid Antibiotics |
Issue Date: | 10-Jul-2014 |
Publisher: | American Society for Biochemistry and Molecular Biology |
Abstract: | We have recently shown that RaaS (regulator of antimicrobial-assisted survival), encoded by Rv1219c in Mycobacterium tuberculosis and by bcg_1279c in Mycobacterium bovis bacillus Calmette-Guérin, plays an important role in mycobacterial survival in prolonged stationary phase and during murine infection. Here, we demonstrate that long chain acyl-CoA derivatives (oleoyl-CoA and, to lesser extent, palmitoyl-CoA) modulate RaaS binding to DNA and expression of the downstream genes that encode ATP-dependent efflux pumps. Moreover, exogenously added oleic acid influences RaaS-mediated mycobacterial improvement of survival and expression of the RaaS regulon. Our data suggest that long chain acyl-CoA derivatives serve as biological indicators of the bacterial metabolic state. Dysregulation of efflux pumps can be used to eliminate non-growing mycobacteria. |
Note: | Versió postprint del document publicat a: http://dx.doi.org/10.1074/jbc.M114.577338 |
It is part of: | Journal of Biological Chemistry, 2014, vol. 289, num. 36, p. 25241-25249 |
URI: | http://hdl.handle.net/2445/62005 |
Related resource: | http://dx.doi.org/10.1074/jbc.M114.577338 |
ISSN: | 0021-9258 |
Appears in Collections: | Articles publicats en revistes (Fonaments Clínics) |
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