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|Title:||Oleoyl Coenzyme A regulates interaction of transcriptional regulator RaaS (Rv1219c) with DNA in Mycobacteria|
Waddell, S. J.
Sarybaeva, A. A.
Tudó i Vilanova, Griselda
Young, D. I.
Andrew, P. W.
Butcher, P. D.
Cohen Gonsaun, M.
|Publisher:||American Society for Biochemistry and Molecular Biology|
|Abstract:||We have recently shown that RaaS (regulator of antimicrobial-assisted survival), encoded by Rv1219c in Mycobacterium tuberculosis and by bcg_1279c in Mycobacterium bovis bacillus Calmette-Guérin, plays an important role in mycobacterial survival in prolonged stationary phase and during murine infection. Here, we demonstrate that long chain acyl-CoA derivatives (oleoyl-CoA and, to lesser extent, palmitoyl-CoA) modulate RaaS binding to DNA and expression of the downstream genes that encode ATP-dependent efflux pumps. Moreover, exogenously added oleic acid influences RaaS-mediated mycobacterial improvement of survival and expression of the RaaS regulon. Our data suggest that long chain acyl-CoA derivatives serve as biological indicators of the bacterial metabolic state. Dysregulation of efflux pumps can be used to eliminate non-growing mycobacteria.|
|Note:||Versió postprint del document publicat a: http://dx.doi.org/10.1074/jbc.M114.577338|
|It is part of:||Journal of Biological Chemistry, 2014, vol. 289, num. 36, p. 25241-25249|
|Appears in Collections:||Articles publicats en revistes (Fonaments Clínics)|
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