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Title: Oleoyl Coenzyme A regulates interaction of transcriptional regulator RaaS (Rv1219c) with DNA in Mycobacteria
Author: Turapov, Obolbek
Waddell, Simon J.
Burke, Bernard
Glenn, Sarah
Sarybaeva, Asel A.
Tudó i Vilanova, Griselda
Labesse, Gilles
Young, Danielle I.
Young, Michael
Andrew, Peter W.
Butcher, Philip D.
Cohen Gonsaun, Martin
Mukamolova, Galina
Keywords: Mycobacterium tuberculosis
Àcid oleic
Mycobacterium tuberculosis
Oleic acid
Issue Date: 10-Jul-2014
Publisher: American Society for Biochemistry and Molecular Biology
Abstract: We have recently shown that RaaS (regulator of antimicrobial-assisted survival), encoded by Rv1219c in Mycobacterium tuberculosis and by bcg_1279c in Mycobacterium bovis bacillus Calmette-Guérin, plays an important role in mycobacterial survival in prolonged stationary phase and during murine infection. Here, we demonstrate that long chain acyl-CoA derivatives (oleoyl-CoA and, to lesser extent, palmitoyl-CoA) modulate RaaS binding to DNA and expression of the downstream genes that encode ATP-dependent efflux pumps. Moreover, exogenously added oleic acid influences RaaS-mediated mycobacterial improvement of survival and expression of the RaaS regulon. Our data suggest that long chain acyl-CoA derivatives serve as biological indicators of the bacterial metabolic state. Dysregulation of efflux pumps can be used to eliminate non-growing mycobacteria.
Note: Versió postprint del document publicat a:
It is part of: Journal of Biological Chemistry, 2014, vol. 289, num. 36, p. 25241-25249
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ISSN: 0021-9258
Appears in Collections:Articles publicats en revistes (Fonaments Clínics)

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