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http://hdl.handle.net/2445/63186
Title: | The L-type voltage-gated calcium channel modulates microglial pro-inflammatory activity |
Author: | Espinosa Parrilla, Juan Francisco Martínez Moreno, Martín Gasull Casanova, Xavier Mahy Gehenne, Josette Nicole Rodríguez Allué, Manuel José |
Keywords: | Micròglia Canals de calci Sistema nerviós central Malalties neurodegeneratives Microglia Calcium channels Central nervous system Neurodegenerative Diseases |
Issue Date: | 12-Dec-2015 |
Publisher: | Elsevier B.V. |
Abstract: | Under pathological conditions, microglia, the resident CNS immune cells, become reactive and release pro-inflammatory cytokines and neurotoxic factors. We investigated whether this phenotypic switch includes changes in the expression of the L-type voltage-gated calcium channel (VGCC) in a rat model of N-methyl-d-aspartate-induced hippocampal neurodegeneration. Double immunohistochemistry and confocal microscopy evidenced that activated microglia express the L-type VGCC. We then analyzed whether BV2 microglia express functional L-type VGCC, and investigated the latter's role in microglial cytokine release and phagocytic capacity. Activated BV2 microglia express the CaV1.2 and CaV1.3 subunits of the L-type VGCC determined by reverse transcription-polymerase chain reaction, Western blot and immunocytochemistry. Depolarization with KCl induced a Ca2+ entry facilitated by Bay k8644 and partially blocked with nifedipine, which also reduced TNF-α and NO release by 40%. However, no nifedipine effect on BV2 microglia viability or phagocytic capacity was observed. Our results suggest that in CNS inflammatory processes, the L-type VGCC plays a specific role in the control of microglial secretory activity. |
Note: | Versió postprint del document publicat a: http://dx.doi.org/10.1016/j.mcn.2014.12.004 |
It is part of: | Molecular and Cellular Neuroscience, 2015, vol. 64, p. 104-115 |
URI: | http://hdl.handle.net/2445/63186 |
Related resource: | http://dx.doi.org/10.1016/j.mcn.2014.12.004 |
ISSN: | 1044-7431 |
Appears in Collections: | Articles publicats en revistes (Ciències Fisiològiques) |
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