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Title: The L-type voltage-gated calcium channel modulates microglial pro-inflammatory activity
Author: Espinosa Parrilla, J. F.
Martínez-Moreno, M.
Gasull Casanova, Xavier
Mahy Gehenne, Josette Nicole
Rodríguez Allué, Manuel José
Keywords: Micròglia
Canals de calci
Sistema nerviós central
Malalties neurodegeneratives
Calcium channels
Central nervous system
Neurodegenerative diseases
Issue Date: 12-Dec-2015
Publisher: Elsevier B.V.
Abstract: Under pathological conditions, microglia, the resident CNS immune cells, become reactive and release pro-inflammatory cytokines and neurotoxic factors. We investigated whether this phenotypic switch includes changes in the expression of the L-type voltage-gated calcium channel (VGCC) in a rat model of N-methyl-d-aspartate-induced hippocampal neurodegeneration. Double immunohistochemistry and confocal microscopy evidenced that activated microglia express the L-type VGCC. We then analyzed whether BV2 microglia express functional L-type VGCC, and investigated the latter's role in microglial cytokine release and phagocytic capacity. Activated BV2 microglia express the CaV1.2 and CaV1.3 subunits of the L-type VGCC determined by reverse transcription-polymerase chain reaction, Western blot and immunocytochemistry. Depolarization with KCl induced a Ca2+ entry facilitated by Bay k8644 and partially blocked with nifedipine, which also reduced TNF-α and NO release by 40%. However, no nifedipine effect on BV2 microglia viability or phagocytic capacity was observed. Our results suggest that in CNS inflammatory processes, the L-type VGCC plays a specific role in the control of microglial secretory activity.
Note: Versió postprint del document publicat a:
It is part of: Molecular and Cellular Neuroscience, 2015, vol. 64, p. 104-115
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ISSN: 1044-7431
Appears in Collections:Articles publicats en revistes (Ciències Fisiològiques)

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