Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/65823
Title: A monolithic glass chip for active single-cell sorting based on mechanical phenotyping
Author: Faigle, C.
Lautenschläger, F.
Whyte, G.
Homewood, P.
Martín Badosa, Estela
Guck, J.
Keywords: Fluorescència
Instruments òptics
Cèl·lules
Fluorescence
Optical instruments
Cells
Issue Date: 16-Dec-2014
Publisher: Royal Society of Chemistry
Abstract: The mechanical properties of biological cells have long been considered as inherent markers of biological function and disease. However, the screening and active sorting of heterogeneous populations based on serial single-cell mechanical measurements has not been demonstrated. Here we present a novel monolithic glass chip for combined fluorescence detection and mechanical phenotyping using an optical stretcher. A new design and manufacturing process, involving the bonding of two asymmetrically etched glass plates, combines exact optical fiber alignment, low laser damage threshold and high imaging quality with the possibility of several microfluidic inlet and outlet channels. We show the utility of such a custombuilt optical stretcher glass chip by measuring and sorting single cells in a heterogeneous population based on their different mechanical properties and verify sorting accuracy by simultaneous fluorescence detection. This offers new possibilities of exact characterization and sorting of small populations based on rheological properties for biological and biomedical applications.
Note: Reproducció del document publicat a: http://dx.doi.org/10.1039/c4lc01196a
It is part of: Lab On a Chip, 2015, vol. 15, num. 5, p. 1267-1275
Related resource: http://dx.doi.org/10.1039/c4lc01196a
URI: http://hdl.handle.net/2445/65823
ISSN: 1473-0197
Appears in Collections:Articles publicats en revistes (Física Aplicada)

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