Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/7303
Title: In vitro susceptibility to pentavalent antimony in Leishmania infantum strains is not modified during in vitro or in vivo passages but is modified after host treatment with meglumine antimoniate
Author: Carrió Díaz-Meco, Jaume
Portús Vinyeta, Montserrat
Keywords: Resistència als medicaments
Leishmaniosi
Drug Resistance
Leishmaniasis
Issue Date: 2002
Publisher: BioMed Central
Abstract: Background: Leishmaniasis is a common parasitic disease in Southern Europe, caused by Leishmania infantum. The failures of current treatment with pentavalent antimonials are partially attributable to the emergence of antimony-resistant Leishmania strains. This study analyses the in vitro susceptibility to pentavalent antimony of intracellular amastigotes from a range of L. infantum strains, derived from the same infected animal, during in vitro and in vivo passages and after host treatment with meglumine antimoniate. Results: SbV-IC50 values for strains from two distinct isolates from the same host and one stock after two years of culture in NNN medium and posterior passage to hamster were similar (5.0 ± 0.2; 4.9 ± 0.2 and 4.4 ± 0.1 mgSbV/L, respectively). In contrast, a significant difference (P < 0.01, t test) was observed between the mean SbV-IC50 values in the stocks obtained before and after treatment of hosts with meglumine antimoniate (4.7 ± 0.4 mgSbV/L vs. 7.7 ± 1.5 mgSbV/L). Drug-resistance after drug pressure in experimentally infected dogs increased over repeated drug administration (6.4 ± 0.5 mgSbV/L after first treatment vs. 8.6 ± 1.4 mgSbV/L after the second) (P < 0.01, t test). Conclusions: These results confirm previous observations on strains from Leishmania/HIV co-infected patients and indicate the effect of the increasing use of antimony derivatives for treatment of canine leishmaniasis in endemic areas on the emergence of Leishmania antimony-resistant strains.
Note: Reproducció del document publicat a http://dx.doi.org/10.1186/1471-2210-2-11
It is part of: BMC Pharmacology, 2002, vol. 2, núm. 11
URI: http://hdl.handle.net/2445/7303
ISSN: 1471-2210
Appears in Collections:Articles publicats en revistes (Biologia, Sanitat i Medi Ambient)

Files in This Item:
File Description SizeFormat 
501553.pdf223.57 kBAdobe PDFView/Open


This item is licensed under a Creative Commons License Creative Commons