Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/97065
Title: Erythrocytes and cell line-based assays to evaluate the cytoprotective activity of antioxidant components obtained from natural sources
Author: Botta, Albert
Martínez Ocaña, Verónica
Mitjans Arnal, Montserrat
Balboa, Elena M.
Conde, Enma
Vinardell Martínez-Hidalgo, Ma. Pilar
Keywords: Antioxidants
Plantes medicinals
Estrès oxidatiu
Hemòlisi
Toxicologia
Farmacologia
Antioxidants
Medicinal plants
Oxidative stress
Hemolysis
Toxicology
Pharmacology
Issue Date: Feb-2014
Publisher: Elsevier Ltd
Abstract: Oxidative stress can damage cellular components including DNA, proteins or lipids, and may cause several skin diseases. To protect from this damage and addressing consumer's appeal to natural products, antioxidants obtained from algal and vegetal extracts are being proposed as antioxidants to be incorporated into formulations. Thus, the development of reliable, quick and economic in vitro methods to study the cytoactivity of these products is a meaningful requirement. A combination of erythrocyte and cell line-based assays was performed on two extracts from Sargassum muticum, one from Ulva lactuca, and one from Castanea sativa. Antioxidant properties were assessed in erythrocytes by the TBARS and AAPH assays, and cytotoxicity and antioxidant cytoprotection were assessed in HaCaT and 3T3 cells by the MTT assay. The extracts showed no antioxidant activity on the TBARS assay, whereas their antioxidant capacity in the AAPH assay was demonstrated. On the cytotoxicity assays, extracts showed low toxicity, with IC50 values higher than 200 µg/mL. C. sativa extract showed the most favourable antioxidant properties on the antioxidant cytoprotection assays; while S. muticum and U. lactuca extracts showed a low antioxidant activity. This battery of methods was useful to characterize the biological antioxidant properties of these natural extracts.
Note: Versió postprint del document publicat a: http://dx.doi.org/10.1016/j.tiv.2013.10.004
It is part of: Toxicology in Vitro, 2014, vol. 28, num. 1, p. 120-124
Related resource: http://dx.doi.org/10.1016/j.tiv.2013.10.004
URI: http://hdl.handle.net/2445/97065
ISSN: 0887-2333
Appears in Collections:Articles publicats en revistes (Bioquímica i Fisiologia)

Files in This Item:
File Description SizeFormat 
629785.pdf673.37 kBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.