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Title: Carbapenem-resistant and carbapenem-susceptible isogenic isolates of Klebsiella pneumoniae ST101 causing infection in a tertiary hospital
Author: Cubero, Meritxell
Cuervo Requena, Guillermo
Domínguez Luzón, Ma. Ángeles (María Ángeles)
Tubau, Fe
Martí Martí, Sara
Sevillano, Elena
Gallego, Lucía
Ayats, Josefina
Peña Miralles, Carmen
Pujol Rojo, Miquel
Liñares Louzao, Josefina
Ardanuy Tisaire, María Carmen
Keywords: Antibiòtics betalactàmics
Klebsiella pneumoniae
Resistència als medicaments
Medicaments antibacterians
Beta lactam antibiotics
Klebsiella pneumoniae
Drug resistance
Antibacterial agents
Issue Date: 3-Sep-2015
Publisher: BioMed Central
Abstract: Background: In this study we describe the clinical and molecular characteristics of an outbreak due to carbapenem-resistant Klebsiella pneumoniae (CR-KP) producing CTX-M-15 and OXA-48 carbapenemase. Isogenic strains, carbapenem-susceptible K. pneumoniae (CS-KP) producing CTX-M-15, were also involved in the outbreak. Results: From October 2010 to December 2012 a total of 62 CR-KP and 23 CS-KP were isolated from clinical samples of 42 patients (22 had resistant isolates, 14 had susceptible isolates, and 6 had both CR and CS isolates). All patients had underlying diseases and 17 of them (14 patients with CR-KP and 3 with CS-KP) had received carbapenems previously. The range of carbapenem MICs for total isolates were: imipenem: 2 to >32 μg/ml vs. <2 μg/ml; meropenem: 4 to >32 μg/ml vs. <2 μg/ml; and ertapenem: 8 to >32 μg/ml vs. <2 μg/ml. All the isolates were also resistant to gentamicin, ciprofloxacin, and cotrimoxazole. Both types of isolates shared a common PFGE pattern associated with the multilocus sequence type 101 (ST101). The blaCTX-M-15 gene was detected in all the isolates, whereas the bla OXA-48 gene was only detected in CR-KP isolates on a 70 kb plasmid. Conclusions: The clonal spread of K. pneumoniae ST101 expressing the OXA-48 and CTX-M-15 beta-lactamases was the cause of an outbreak of CR-KP infections. CTX-M-15-producing isolates lacking the bla OXA-48 gene coexisted during the outbreak.
Note: Reproducció del document publicat a:
It is part of: BMC Microbiology, 2015, vol. 15, p. 177
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ISSN: 1471-2180
Appears in Collections:Articles publicats en revistes (Patologia i Terapèutica Experimental)
Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))

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