Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/99252
Title: An Antibody Screen of a Plasmodium vivax Antigen Library Identifies Novel Merozoite Proteins Associated with Clinical Protection
Author: Franca, Camila T.
Hostetler, Jessica B.
Sharma, Sumana
White, Michael T.
Lin, Enmoore
Kiniboro, Benson
Waltmann, Andreea
Darcy, Andrew W.
Li Wai Suen, Connie S. N.
Siba, Peter
King, Christopher L.
Rayner, Julian C.
Fairhurst, Rick M.
Mueller, Ivo
Keywords: Plasmodium vivax
Malària
Plasmodium vivax
Malaria
Issue Date: 16-May-2016
Publisher: Public Library of Science (PLoS)
Abstract: BACKGROUND: Elimination of Plasmodium vivax malaria would be greatly facilitated by the development of an effective vaccine. A comprehensive and systematic characterization of antibodies to P. vivax antigens in exposed populations is useful in guiding rational vaccine design. METHODOLOGY/PRINCIPAL FINDINGS: In this study, we investigated antibodies to a large library of P. vivax entire ectodomain merozoite proteins in 2 Asia-Pacific populations, analysing the relationship of antibody levels with markers of current and cumulative malaria exposure, and socioeconomic and clinical indicators. 29 antigenic targets of natural immunity were identified. Of these, 12 highly-immunogenic proteins were strongly associated with age and thus cumulative lifetime exposure in Solomon Islanders (P<0.001-0.027). A subset of 6 proteins, selected on the basis of immunogenicity and expression levels, were used to examine antibody levels in plasma samples from a population of young Papua New Guinean children with well-characterized individual differences in exposure. This analysis identified a strong association between reduced risk of clinical disease and antibody levels to P12, P41, and a novel hypothetical protein that has not previously been studied, PVX_081550 (IRR 0.46-0.74; P<0.001-0.041). CONCLUSION/SIGNIFICANCE: These data emphasize the benefits of an unbiased screening approach in identifying novel vaccine candidate antigens. Functional studies are now required to establish whether PVX_081550 is a key component of the naturally-acquired protective immune response, a biomarker of immune status, or both.
Note: Reproducció del document publicat a: http://dx.doi.org/10.1371/journal.pntd.0004639
It is part of: PLoS Neglected Tropical Diseases, 2016, vol. 10, num. 5, p. e0004639
Related resource: http://dx.doi.org/10.1371/journal.pntd.0004639
URI: http://hdl.handle.net/2445/99252
ISSN: 1935-2727
Appears in Collections:Articles publicats en revistes (ISGlobal)

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