Dipòsit Digital de la Universitat de Barcelona
El Dipòsit Digital de la Universitat de Barcelona és el repositori institucional que conté en format digital els materials derivats de l'activitat docent, investigadora i institucional de la comunitat universitària.
Enviaments recents
ArticleTwo-layer elastographic 3-D traction force microscopy
(Nature Publishing Group, 2017-01-11) Álvarez-González, Begoña; Zhang, Shun; Gómez González, Manuel; Meili, Ruedi; Firtel, Richard A.; Lasheras, Juan C.; del Álamo, Juan C.
Cellular traction force microscopy (TFM) requires knowledge of the mechanical properties of the substratum where the cells adhere to calculate cell-generated forces from measurements of substratum deformation. Polymer-based hydrogels are broadly used for TFM due to their linearly elastic behavior in the range of measured deformations. However, the calculated stresses, particularly their spatial patterns, can be highly sensitive to the substratum’s Poisson’s ratio. We present two-layer elastographic TFM (2LETFM), a method that allows for simultaneously measuring the Poisson’s ratio of the substratum while also determining the cell-generated forces. The new method exploits the analytical solution of the elastostatic equation and deformation measurements from two layers of the substratum. We perform an in silico analysis of 2LETFM concluding that this technique is robust with respect to TFM experimental parameters, and remains accurate even for noisy measurement data. We also provide experimental proof of principle of 2LETFM by simultaneously measuring the stresses exerted by migrating Physarum amoeboae on the surface of polyacrylamide substrata, and the Poisson’s ratio of the substrata. The 2LETFM method could be generalized to concurrently determine the mechanical properties and cell-generated forces in more physiologically relevant extracellular environments, opening new possibilities to study cell-matrix interactions.
Article'Neoliberalismo y postfeminismo en Revolt. She Said. Revolt Again. de Alice Birch: Un manifiesto feminista para el siglo XXI'
(Universidad de La Laguna, 2020) Escoda, Clara
Este artículo se inscribe en el proyecto British Theatre in the Twenty First Century: Crisis, Affect, Community (FFI2016‑75443) y constituye una de las cuatro aportaciones que desarrollé en el marco de esta investigación sobre afectos, neoliberalismo y subjetividad política. El estudio analiza Revolt. She Said. Revolt Again de Alice Birch desde la teoría de los afectos y el feminismo contemporáneo, y examina cómo la obra denuncia los discursos neoliberales y posfeministas que promueven el individualismo, la autosuficiencia y la “inmunidad” frente al otro. A partir del concepto de inmunidad de Jean‑Luc Nancy, el capítulo muestra cómo Birch resiste estos discursos deshumanizantes y propone, en su lugar, una ética feminista basada en el cuidado, la interdependencia y la responsabilidad afectiva.El capítulo constituye, además, la primera lectura que aplica de manera sistemática el concepto de “inmunidad” de Jean‑Luc Nancy al análisis de Revolt. She Said. Revolt Again, situando la obra en el marco del giro afectivo y del neoliberalismo emocional. Esta aportación pionera ilumina la crítica que Birch dirige a las economías afectivas del capitalismo tardío y propone una relectura feminista de la agencia política basada en el cuidado y la vulnerabilidad compartida. Con ello, el artículo abre una línea de investigación que no había sido desarrollada previamente en los estudios sobre la dramaturga.El artículo destaca la relevancia de Birch como una de las voces más innovadoras del teatro británico contemporáneo y evidencia mi interés sostenido por la dramaturgia escrita por mujeres y por los efectos del neoliberalismo sobre los cuerpos —especialmente los cuerpos feminizados— en el siglo XXI. Este trabajo forma parte de una línea de investigación consolidada que desarrollé en paralelo en estudios sobre Martin Crimp y Kae Tempest, y que demuestra la amplitud, coherencia y calidad de mi aportación al proyecto.Asimismo, este artículo anticipa los ejes temáticos de mi proyecto posterior como investigadora principal, Gender, Affect and Care in 21st Century British Theatre (PID2021‑126448NA‑I00), centrado en el papel del afecto, el género y las prácticas de cuidado en la escena contemporánea. Esta trayectoria culmina en el volumen Care Matters in 21st Century British Theatre (Routledge, forthcoming), que coedito junto con Verónica Rodríguez y José Ramón Prado Pérez. En conjunto, este artículo constituye un paso decisivo en la articulación de una línea de investigación propia sobre afectos, feminismo y ética del cuidado en el teatro británico actual.
Article Role of PATJ in stroke prognosis by modulating endothelial to mesenchymal transition through the Hippo/Notch/PI3K axis.
(2024-02-17) Medina-Dols, Aina; Cañellas, Guillem; Capó, Toni; Solé, Montse; Mola Caminal, Marina; Cullell, Natalia; Jaume, Marina; Nadal-Salas, Laura; Llinàs, Jaume; Gómez, Lluís; Tur, Silvia; Jimenez, Carmen; Diaz-Navarro, Rosa M.; Carrera, Caty; Muiño Acuña, Elena; Gallego Fàbrega, Cristina; Soriano Tarraga, Carolina; Ruiz-Guerra, Laura; Pol-Fuster, Josep; Asensio, Víctor; Muncunill, Josep; Fleischer, Aarne; Iglesias, Amanda; Giralt Steinhauer, Eva; Lazcano, Uxue; Fernández-Pérez, Isabel; Jiménez-Balado, Joan; Gabriel-Salazar, Marina; Garcia-Gabilondo, Miguel; Lei, Ting; Torres Águila, Nuria Paz; Carcel-Marquez, Jara; Lladó, Jerònia; Olmos, Gabriel; Rosell, Anna; Montaner, Joan; Planas Obradors, Anna Maria; Rabionet Janssen, Raquel; Hernandez-Guillamon, Mar; Jiménez Conde, Jordi; Fernandez-Cadenas, Israel; Vives-Bauzà, Cristòfol
Through GWAS studies we identified PATJ associated with functional outcome after ischemic stroke (IS). The aim of this study was to determine PATJ role in brain endothelial cells (ECs) in the context of stroke outcome. PATJ expression analyses in patient’s blood revealed that: (i) the risk allele of rs76221407 induces higher expression of PATJ, (ii) PATJ is downregulated 24 h after IS, and (iii) its expression is significantly lower in those patients with functional independence, measured at 3 months with the modified Rankin scale ((mRS) ≤2), compared to those patients with marked disability (mRS = 4–5). In mice brains, PATJ was also downregulated in the injured hemisphere at 48 h after ischemia. Oxygen-glucose deprivation and hypoxia-dependent of Hypoxia Inducible Factor-1α also caused PATJ depletion in ECs. To study the effects of PATJ downregulation, we generated PATJ-knockdown human microvascular ECs. Their transcriptomic profile evidenced a complex cell reprogramming involving Notch, TGF-ß, PI3K/Akt, and Hippo signaling that translates in morphological and functional changes compatible with endothelial to mesenchymal transition (EndMT). PATJ depletion caused loss of cell-cell adhesion, upregulation of metalloproteases, actin cytoskeleton remodeling, cytoplasmic accumulation of the signal transducer C-terminal transmembrane Mucin 1 (MUC1-C) and downregulation of Notch and Hippo signaling. The EndMT phenotype of PATJ-depleted cells was associated with the nuclear recruitment of MUC1-C, YAP/TAZ, β-catenin, and ZEB1. Our results suggest that PATJ downregulation 24 h after IS promotes EndMT, an initial step prior to secondary activation of a pro-angiogenic program. This effect is associated with functional independence suggesting that activation of EndMT shortly after stroke onset is beneficial for stroke recovery.
ArticleEllenberg-type indicator values for European vascular plant species
(Wiley-Blackwell, 2023) Tichý, Lubomír; Axmanová, Irena; Dengler, Jürgen; Guarino, Riccardo; Jansen, Florian; Midolo, Gabriele; Nobis, Michael P.; Van Meerbeek, Koenraad; Acic, Svetlana; Attorre, Fabio; Chytrý, Milan; Díte, Daniel; Díte, Zuzana; Dziuba, Tetiana; Fanelli, Giuliano; Fernández-Pascual, Eduardo; Garbolino, Emmanuel; Gavilán, Rosario G.; Gégout, Jean-Claude; Graf, Ulrich; Güler, Behlül; Hájek, Michal; Hennekens, Stephan M.; Jandt, Ute; Jasková, Anni; Jiménez-Alfaro, Borja; Julve, Philippe; Kambach, Stephan; Karger, Dirk N.; Karrer, Gerhard; Kavgaci, Ali; Knollová, Ilona; Kuzemko, Anna; Küzmic, Filip; Landucci, Flavia; Lengyel, Attila; Lenoir, Jonathan; Marcenò, Corrado; Moeslund, Jesper Erenskjold; Novák, Pavel; Pérez Haase, Aaron; Peterka, Tomás; Pielech, Remigiusz; Pignatti, Alessandro; Rasomavicius, Valerijus; Bergmeier, Erwin; Rusina, Solvita; Biurrun, Idoia; Saatkamp, Arne; Bonari, Gianmaria; Urban Silc; Bruelheide, Helge; Skvorc, Zeljko; Campos, Juan Antonio; Theurillat, Jean-Paul; Carni, Andraz; Wohlgemuth, Thomas; Chiarucci, Alessandro; Cuk, Mirjana; Custerevska, Renata; Didukh, Yakiv
Aims Ellenberg-type indicator values are expert-based rankings of plant species according to their ecological optima on main environmental gradients. Here we extend the indicator-value system proposed by Heinz Ellenberg and co-authors for Central Europe by incorporating other systems of Ellenberg-type indicator values (i.e., those using scales compatible with Ellenberg values) developed for other European regions. Our aim is to create a harmonized data set of Ellenberg-type indicator values applicable at the European scale. Methods We collected European data sets of indicator values for vascular plants and selected 13 data sets that used the nine-, ten- or twelve-degree scales defined by Ellenberg for light, temperature, moisture, reaction, nutrients and salinity. We compared these values with the original Ellenberg values and used those that showed consistent trends in regression slope and coefficient of determination. We calculated the average value for each combination of species and indicator values from these data sets. Based on species' co-occurrences in European vegetation plots, we also calculated new values for species that were not assigned an indicator value. Results We provide a new data set of Ellenberg-type indicator values for 8908 European vascular plant species (8168 for light, 7400 for temperature, 8030 for moisture, 7282 for reaction, 7193 for nutrients, and 7507 for salinity), of which 398 species have been newly assigned to at least one indicator value. Conclusions The newly introduced indicator values are compatible with the original Ellenberg values. They can be used for large-scale studies of the European flora and vegetation or for gap-filling in regional data sets. The European indicator values and the original and taxonomically harmonized regional data sets of Ellenberg-type indicator values are available in the Supporting Information and the Zenodo repository.
ArticleIn-Yer-Face Theatre' and the 'Society of Spectacle': The Violence of Progress in Martin Crimp's The Treatment
(Universitatea din Craiova, 2010) Escoda, Clara
The Treatment was published in 1993, right aftes the fall of Berlín Wall. The tearing down of BerlinWall signified a transition from the social-oriented type of capitalism which has benn practised since the end of the World War TWO, into a tyoe of capitalism which has benn increasingly based on lasissez-faire, neo-liberal policies.