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http://hdl.handle.net/2445/101543
Title: | Sensitive and Rapid UHPLC-MS/MS for the Analysis of Tomato Phenolics in Human Biological Samples |
Author: | Martínez Huélamo, Miriam Tulipani, Sara Jáuregui Pallarés, Olga Valderas Martínez, Palmira Vallverdú i Queralt, Anna Estruch Riba, Ramon Torrado, Xavier Lamuela Raventós, Rosa Ma. |
Keywords: | Biologia humana Tomàquets Metabòlits Human biology Tomatoes Metabolites |
Issue Date: | 2015 |
Publisher: | MDPI |
Abstract: | An UHPLC-MS/MS method for the quantification of tomato phenolic metabolites in human fluids was optimized and validated, and then applied in a pilot dietary intervention study with healthy volunteers. A 5-fold gain in speed (3.5 min of total run); 7-fold increase in MS sensitivity and 2-fold greater efficiency (50% peak width reduction) were observed when comparing the proposed method with the reference-quality HPLC-MS/MS system, whose assay performance has been previously documented. The UHPLC-MS/MS method led to an overall improvement in the limits of detection (LOD) and quantification (LOQ) for all the phenolic compounds studied. The recoveries ranged between 68% and 100% in urine and 61% and 100% in plasma. The accuracy; intra- and interday precision; and stability met with the acceptance criteria of the AOAC International norms. Due to the improvements in the analytical method; the total phenolic metabolites detected in plasma and urine in the pilot intervention study were 3 times higher than those detected by HPLC-MS/MS. Comparing with traditional methods; which require longer time of analysis; the methodology described is suitable for the analysis of phenolic compounds in a large number of plasma and urine samples in a reduced time frame. |
Note: | Reproducció del document publicat a: http://dx.doi.org/10.3390/molecules201119702 |
It is part of: | Molecules, 2015, vol. 20, num. 11, p. 20409-20425 |
URI: | http://hdl.handle.net/2445/101543 |
Related resource: | http://dx.doi.org/10.3390/molecules201119702 |
ISSN: | 1420-3049 |
Appears in Collections: | Articles publicats en revistes (Nutrició, Ciències de l'Alimentació i Gastronomia) |
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