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http://hdl.handle.net/2445/104313| Title: | Elucidation of the biosynthesis of carnosic acid and its reconstitution in yeast |
| Author: | Scheler, Ulschan Brandt, Wolfgang Porzel, Andrea Rothe, Kathleen Manzano Alías, David Bozic, Dragana Papaefthimiou, Dimitra Balcke, Gerd Ulrich Henning, Anja Lohse, Swanhild Marillonnet, Sylvestre Kanellis, Angelos K. Ferrer i Prats, Albert Tissier, Alain |
| Keywords: | Additius alimentaris Llevats (Botànica) Biosíntesi Food additives Yeast fungi Biosynthesis |
| Issue Date: | 5-Oct-2016 |
| Publisher: | Nature Publishing Group |
| Abstract: | Rosemary extracts containing the phenolic diterpenes carnosic acid and its derivative carnosol are approved food additives used in an increasingly wide range of products to enhance shelf-life, thanks to their high anti-oxidant activity. We describe here the elucidation of the complete biosynthetic pathway of carnosic acid and its reconstitution in yeast cells. Cytochrome P450 oxygenases (CYP76AH22-24) from Rosmarinus officinalis and Salvia fruticosa already characterized as ferruginol synthases are also able to produce 11-hydroxyferruginol. Modelling-based mutagenesis of three amino acids in the related ferruginol synthase (CYP76AH1) from S. miltiorrhiza is sufficient to convert it to a 11-hydroxyferruginol synthase (HFS). The three sequential C20 oxidations for the conversion of 11-hydroxyferruginol to carnosic acid are catalysed by the related CYP76AK6-8. The availability of the genes for the biosynthesis of carnosic acid opens opportunities for the metabolic engineering of phenolic diterpenes, a class of compounds with potent anti-oxidant, anti-inflammatory and anti-tumour activities. |
| Note: | Reproducció del document publicat a: https://doi.org/10.1038/ncomms12942 |
| It is part of: | Nature Communications, 2016, vol. 7, p. 1-11 |
| URI: | http://hdl.handle.net/2445/104313 |
| Related resource: | https://doi.org/10.1038/ncomms12942 |
| ISSN: | 2041-1723 |
| Appears in Collections: | Articles publicats en revistes (Bioquímica i Fisiologia) Publicacions de projectes de recerca finançats per la UE |
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