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DC Field | Value | Language |
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dc.contributor.author | Iturbe Espinoza, Paul | - |
dc.contributor.author | Gil Moreno, Selene | - |
dc.contributor.author | Lin, Weiyu | - |
dc.contributor.author | Calatayud, Sara | - |
dc.contributor.author | Palacios Bonilla, Òscar | - |
dc.contributor.author | Capdevila Vidal, Mercè | - |
dc.contributor.author | Atrian i Ventura, Sílvia | - |
dc.date.accessioned | 2016-12-02T16:46:30Z | - |
dc.date.available | 2016-12-02T16:46:30Z | - |
dc.date.issued | 2016-02-16 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | http://hdl.handle.net/2445/104427 | - |
dc.description.abstract | Fungal Cu-thioneins, and among them, the paradigmatic Neurospora crassa metallothio- nein (MT) (26 residues), were once considered as the shortest MTs -the ubiquitous, versa- tile metal-binding proteins- among all organisms, and thus representatives of their primeval forms. Nowadays, fungal MTs of diverse lengths and sequence features are known, follow- ing the huge heterogeneity of the Kingdom of Fungi. At the opposite end of N . crassa MT, the recently reported Cryptococcus neoformans CnMT1 and CnMT2 (122 and 186 aa) con- stitute the longest reported fungal MTs, having been identified as virulence factors of this pathogen. CnMTs are high-capacity Cu-thioneins that appear to be built by tandem amplifi- cation of a basic unit, a 7-Cys segment homologous to N . crassa MT. Here, we report the in silico , in vivo and in vitro study of a still longer fungal MT, belonging to Tremella mesenter- ica (TmMT), a saprophytic ascomycete. The TmMT gene has 10 exons, and it yields a 779-bp mature transcript that encodes a 257 residue-long protein. This MT is also built by repeated fragments, but of variable number of Cys: six units of the 7-Cys building blocks- CXCX 3 CSCPPGXCXCAXCP-, two fragments of six Cys, plus three Cys at the N-terminus. TmMT metal binding abilities have been analyzed through the spectrophotometric and spectrometric characterization of its recombinant Zn-, Cd- and Cu-complexes. Results allow it to be unambiguous classified as a Cu-thionein, also of extraordinary coordinating capacity. According to this feature, when the TmMT cDNA is expressed in MT-devoid yeast cells, it is capable of restoring a high Cu tolerance level. Since it is not obvious that T . mesenterica shares the same physiological needs for a high capacity Cu-binding protein with C . neoformans , the existence of this peculiar MT might be better explained on the basis of a possible role in Cu-handling for the Cu-enzymes responsible in lignin degradation pathways. | - |
dc.format.extent | 22 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Public Library of Science (PLoS) | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.1371/journal.pone.0148651 | - |
dc.relation.ispartof | PLoS One, 2016, vol. 11, num. 2, p. e0148651 | - |
dc.relation.uri | https://doi.org/10.1371/journal.pone.0148651 | - |
dc.rights | cc-by (c) Iturbe Espinoza, Paul. et al., 2016 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es | - |
dc.source | Articles publicats en revistes (Genètica, Microbiologia i Estadística) | - |
dc.subject.classification | Fongs | - |
dc.subject.classification | Fongs patògens | - |
dc.subject.classification | Expressió gènica | - |
dc.subject.other | Fungi | - |
dc.subject.other | Pathogenic fungi | - |
dc.subject.other | Gene expression | - |
dc.title | The fungus Tremella mesenterica encodes the longest metallothionein currently known: gene, protein and metal binding characterization | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 657320 | - |
dc.date.updated | 2016-12-02T16:46:35Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 26882011 | - |
Appears in Collections: | Articles publicats en revistes (Genètica, Microbiologia i Estadística) |
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657320.pdf | 6.23 MB | Adobe PDF | View/Open |
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