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http://hdl.handle.net/2445/104526
Title: | Blockade of the SNARE protein syntaxin 1 inhibits glioblastoma tumor growth |
Author: | Ulloa Darquea, Fausto Alexander Gonzàlez Juncà, Alba Meffre, Delphine Barrecheguren Manero, Pablo José Martínez Mármol, Ramón Pazos Capell, Irene Olivé, Núria Cotrufo, Tiziana Seoane Suárez, Joan Soriano García, Eduardo |
Keywords: | Proteïnes citosquelètiques Tumors Glioma Cytoskeletal proteins Tumors Gliomas |
Issue Date: | 24-Mar-2015 |
Publisher: | Public Library of Science (PLoS) |
Abstract: | Glioblastoma (GBM) is the most prevalent adult brain tumor, with virtually no cure, and with a median overall survival of 15 months from diagnosis despite of the treatment. SNARE pro- teins mediate membrane fusion events in cells and are essential for many cellular process- es including exocytosis and neurotransmission, intracellular trafficking and cell migration. Here we show that the blockade of the SNARE protein Syntaxin 1 (Stx1) function impairs GBM cell proliferation. We show that Stx1 loss-of-function in GBM cells, through ShRNA lentiviral transduction, a Stx1 dominant negative and botulinum toxins, dramatically reduces the growth of GBM after grafting U373 cells into the brain of immune compromised mice. In- terestingly, Stx1 role on GBM progression may not be restricted just to cell proliferation since the blockade of Stx1 also reduces in vitro GBM cell invasiveness suggesting a role in several processes relevant for tumor progression. Altogether, our findings indicate that the blockade of SNARE proteins may represent a novel therapeutic tool against GBM. |
Note: | Reproducció del document publicat a: https://doi.org/10.1371/journal.pone.0119707 |
It is part of: | PLoS One, 2015, vol. 10, num. 3, p. e0119707 |
URI: | http://hdl.handle.net/2445/104526 |
Related resource: | https://doi.org/10.1371/journal.pone.0119707 |
ISSN: | 1932-6203 |
Appears in Collections: | Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia) |
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